FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40795
Sequences flagged as poor quality0
Sequence length36-90
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCC10262.5150140948645667No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA8392.056624586346366No Hit
GGGGATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTC7911.9389631082240473No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC5671.3898762103198923No Hit
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC4551.1153327613678148No Hit
GGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTGC4331.0614045838950852No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT4241.0393430567471504No Hit
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC3750.9192302978306165No Hit
GGGGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCTTGTCCTCTTCTC3570.8751072435347469No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG3560.8726559627405319No Hit
GGGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACT3430.8407893124157372No Hit
GGGGGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCTTGTCCTCTTCT3140.7697021693835029No Hit
GGGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTG2990.7329329574702782No Hit
GGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC2450.6005637945826694No Hit
GGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCAG2310.5662458634636598No Hit
GGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA2230.54663561710994No Hit
GGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAAA2190.53683049393308No Hit
GGGAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTTTTCCCTTCGTC2060.5049638436082854No Hit
GGGGAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTTTTCCCTTCGT1920.4706459124892756No Hit
GGGATAGTTCTCCCTGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCT1890.4632920701066307No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG1840.4510356661355558No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTC1740.426522858193406No Hit
GGGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAA1660.4069126118396863No Hit
GGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA1620.39710748866282636No Hit
GGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTG1610.3946562078686114No Hit
GGGAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCTG1580.3873023654859664No Hit
GGGCAAGGATTACATCGCCCTGAACGAGGACCTGCGCTCCTGGACCGCCG1530.3750459615148915No Hit
GGGCGCCTCCTCCGCGGGTATGACCAGTCCGCCTACGACGGCAAGGATTA1510.37014339992646156No Hit
GGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG1490.36524083833803167No Hit
GGGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA1450.35543571516117173No Hit
GGGGATAGTTCTCCCTGATTGAGACTTGCCTGCTGCTGTGACCACTGGTC1400.3431793111900968No Hit
GGGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCT1350.33092290721902196No Hit
GGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTG1340.328471626424807No Hit
GGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTC1310.321117784042162No Hit
GGGGGGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCC1300.31866650324794704No Hit
GGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC1230.3015075376884422No Hit
GGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAG1140.27944601054050744No Hit
GGGGGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCT1120.2745434489520775No Hit
GGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAA1110.2720921681578625No Hit
GGGGAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCT1110.2720921681578625No Hit
GGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGC1100.2696408873636475No Hit
GGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGG1010.24757936021571272No Hit
GGGCTCACAATTACTCTACAGCTCAGAACAGCAACTGCTGAGGCTGCCTT920.2255178330677779No Hit
GGGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCC870.213261429096703No Hit
GGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG870.213261429096703No Hit
GGGCCCTATAATTTGGAATGTGGGTGGAGAGGGGTCATAGTTCTCCCTGA860.21081014830248804No Hit
GGGGGAGAGGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTG850.20835886750827307No Hit
GGGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTG840.20590758671405807No Hit
GGGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTT820.20100502512562815No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG780.19119990194876824No Hit
GGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCT760.18629734036033826No Hit
GGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACCC750.1838460595661233No Hit
GGGGGGAGAGGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCT720.17649221718347838No Hit
GGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTT710.1740409363892634No Hit
GGGGCCCTTTCATCAGTCTTCCCGCGTCCGCCGATTCCTCCTCCTTGGTC710.1740409363892634No Hit
GGGCTTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAAA660.1617845324181885No Hit
GGGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCT640.15688197082975855No Hit
GGGCCCTTTCATCAGTCTTCCCGCGTCCGCCGATTCCTCCTCCTTGGTCG630.15443069003554358No Hit
GGGATTCTCTTTTCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTC610.1495281284471136No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA610.1495281284471136No Hit
GGGCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGC600.14707684765289863No Hit
GGGGATATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGG570.13972300527025372No Hit
GGGGGAATGTGGGTGGAGAGGGGTCATAGTTCTCCCTGAGTGAGACTCAC560.13727172447603875No Hit
GGGCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCC560.13727172447603875No Hit
GGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG550.13482044368182375No Hit
GGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGTT550.13482044368182375No Hit
GGGGGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCT550.13482044368182375No Hit
GGGATATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGT550.13482044368182375No Hit
GGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGT550.13482044368182375No Hit
GGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCA550.13482044368182375No Hit
GGGGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCTC530.1299178820933938No Hit
GGGACTCCCGAGCTCTACTGACTCCCAAAAGAGCGCCCAAGAAGAAAATG520.12746660129917883No Hit
GGGGCTCACAATTACTCTACAGCTCAGAACAGCAACTGCTGAGGCTGCCT520.12746660129917883No Hit
GGGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACC520.12746660129917883No Hit
GGGCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTGTGCTCACAGTCA510.12501532050496383No Hit
GGGCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGC510.12501532050496383No Hit
GGGCAAGGATTACATCGCCCTGAAAGAGGACCTGCGCTCTTGGACCGCGG510.12501532050496383No Hit
GGGATAATTTGGAATGTGGGTGGAGAGGGGTCATAGTTCTCCCTGAGTGA510.12501532050496383No Hit
GGGTATGACCAGTCCGCCTACGACGGCAAGGATTACATCGCCCTGAACGA510.12501532050496383No Hit
GGGGCCCTATAATTTGGAATGTGGGTGGAGAGGGGTCATAGTTCTCCCTG500.12256403971074886No Hit
GGGTCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTC500.12256403971074886No Hit
GGGGTCCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGA500.12256403971074886No Hit
GGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTGGTGC490.12011275891653389No Hit
GGGAGTAACTTCTTCCCTATAATTTGGAATGTGGGTGGAGAGGGGTCATA480.1176614781223189No Hit
GGGCCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAG480.1176614781223189No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG470.11521019732810393No Hit
GGGGGTCTTGTCCTCTTCTCCAGCATGGTGTGTCTGAAGCTCCCTGGAGG470.11521019732810393No Hit
GGGTCCTCTGGCCCCTGGTCCTGTCCTGTTCTCCAGCATGGTGTGTCTGA460.11275891653388895No Hit
GGGGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTC460.11275891653388895No Hit
GGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGA460.11275891653388895No Hit
GGGGCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAG460.11275891653388895No Hit
GGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGGCCTC450.11030763573967398No Hit
GGGGGTCCTGTCCTGTTCTCCAGCATGGTGTGTCTGAAGCTCCCTGGAGG450.11030763573967398No Hit
GGGGCTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTT450.11030763573967398No Hit
GGGCATCAGGTCCGAGCTGTGTTGACTACCACTTTTCCCTTCGTCTCAAT450.11030763573967398No Hit
GGGAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTCCA440.10785635494545899No Hit
GGGGATAATTTGGAATGTGGGTGGAGAGGGGTCATAGTTCTCCCTGAGTG430.10540507415124402No Hit
GGGGAGAGGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGG430.10540507415124402No Hit
GGGGAGTTCTCCCTGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCTT430.10540507415124402No Hit
GGGCCATCAGGTCCGAGCTGTGTTGACTACCACTTTTCCCTTCGTCTCAA420.10295379335702903No Hit
GGGGAGTAACTTCTTCCCTATAATTTGGAATGTGGGTGGAGAGGGGTCAT420.10295379335702903No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC420.10295379335702903No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)