FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12606
Sequences flagged as poor quality0
Sequence length36-90
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT2742.1735681421545294No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCC2221.7610661589719183No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC2051.6262097413929877No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA1851.4675551324765985No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTG1771.4040932889100428No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTC1691.340631445343487No Hit
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC1511.1978422973187373No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCC1501.1899095668729176No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTG1471.1661113755354593No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC1461.1581786450896399No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTC1361.0788513406314455No Hit
GGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCAG1351.0709186101856258No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA1301.0312549579565287No Hit
GGGGAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCT1210.9598603839441536No Hit
GGGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACT1200.9519276534983341No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG1190.9439949230525148No Hit
GGGGAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCT1150.9122640012692369No Hit
GGGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA920.7298112010153895No Hit
GGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA840.666349357448834No Hit
GGGGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTGT820.6504838965571951No Hit
GGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTG770.6108202443280977No Hit
GGGGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGT760.6028875138822782No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT730.5790893225448199No Hit
GGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCCTCCTGCTGC640.5076947485324449No Hit
GGGGGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTG600.47596382674916704No Hit
GGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAAA590.4680310963033476No Hit
GGGCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT590.4680310963033476No Hit
GGGGGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTG570.4521656354117087No Hit
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC530.42043471362843093No Hit
GGGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTT510.404569252736792No Hit
GGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTG510.404569252736792No Hit
GGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTC500.39663652229097257No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCC500.39663652229097257No Hit
GGGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCT450.3569728700618753No Hit
GGGCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC440.34904013961605584No Hit
GGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAACA440.34904013961605584No Hit
GGGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCCTCCTGCTG430.3411074091702364No Hit
GGGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCC380.3014437569411391No Hit
GGGGGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGTCCTGTTCT380.3014437569411391No Hit
GGGGGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCT370.2935110264953197No Hit
GGGATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCA350.2776455656036808No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG350.2776455656036808No Hit
GGGGCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTC340.2697128351578614No Hit
GGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACCC330.2617801047120419No Hit
GGGCTCACAATTACTCTACAGCTCAGAACACCAACTGCTGAGGCTGCCTT330.2617801047120419No Hit
GGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG310.24591464382040296No Hit
GGGGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGTCCTGTTCTC310.24591464382040296No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTC300.23798191337458352No Hit
GGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC300.23798191337458352No Hit
GGGGGGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCC290.23004918292876408No Hit
GGGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACC290.23004918292876408No Hit
GGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA270.21418372203712518No Hit
GGGCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGC270.21418372203712518No Hit
GGGAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTC270.21418372203712518No Hit
GGGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAA260.20625099159130572No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG260.20625099159130572No Hit
GGGAGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGT260.20625099159130572No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG260.20625099159130572No Hit
GGGCCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAG260.20625099159130572No Hit
GGGGGAATGTGGGTGGAGGGGTTCATAGTTCTCCCTGAGTGAGACTTGCC250.19831826114548629No Hit
GGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACT250.19831826114548629No Hit
GGGATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTCGTCTCA240.19038553069966682No Hit
GGGGAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTT240.19038553069966682No Hit
GGGCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTCGTCT240.19038553069966682No Hit
GGGGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTGTCCTGTTCTC200.15865460891638902No Hit
GGGGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTC200.15865460891638902No Hit
GGGGGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTGTCCTCTTCT200.15865460891638902No Hit
GGGGCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTCGTC190.15072187847056956No Hit
GGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC190.15072187847056956No Hit
GGGGCTCACAATTACTCTACAGCTCAGAACACCAACTGCTGAGGCTGCCT190.15072187847056956No Hit
GGGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGAC190.15072187847056956No Hit
GGGGCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT180.14278914802475012No Hit
GGGGCTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGG180.14278914802475012No Hit
GGGGCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAG180.14278914802475012No Hit
GGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGACT180.14278914802475012No Hit
GGGAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTCCA180.14278914802475012No Hit
GGGGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGTC180.14278914802475012No Hit
GGGTCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTC170.1348564175789307No Hit
GGGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAC170.1348564175789307No Hit
GGGGAGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCG170.1348564175789307No Hit
GGGGAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTT170.1348564175789307No Hit
GGGAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTC170.1348564175789307No Hit
GGGATTCTCTTTTCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTC170.1348564175789307No Hit
GGGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGAC160.12692368713311122No Hit
GGGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCT160.12692368713311122No Hit
GGGCTTTTCACAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCCT160.12692368713311122No Hit
GGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGC160.12692368713311122No Hit
GGGCAAGGATTACATCGCCCTGAAAGAGGACCTGCGCTCTTGGACCGCGG160.12692368713311122No Hit
GGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG160.12692368713311122No Hit
GGGGATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTCGTCTC150.11899095668729176No Hit
GGGGCCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAA150.11899095668729176No Hit
GGGGGCTTCTCTGGCCCCTGGTCCTGTCCTGTTCTCCAGCATGGTGTGTC150.11899095668729176No Hit
GGGAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGTCCTCCTGCTGC150.11899095668729176No Hit
GGGCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTCGTCTC150.11899095668729176No Hit
GGGGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTT150.11899095668729176No Hit
GGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTT140.11105822624147231No Hit
GGGGGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTT140.11105822624147231No Hit
GGGCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGC140.11105822624147231No Hit
GGGGAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGTCCTCCTGCTG140.11105822624147231No Hit
GGGAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCC140.11105822624147231No Hit
GGGGGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTGTCCTGTTCT140.11105822624147231No Hit
GGGAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCAATT140.11105822624147231No Hit
GGGGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTGTCCTCTTCTC130.10312549579565286No Hit
GGGACATCAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTC130.10312549579565286No Hit
GGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAG130.10312549579565286No Hit
GGGGGCTGCTCTGGCCCCTGGTCCTGTCCTGTTCTCCAGCATGGTGTGTC130.10312549579565286No Hit
GGGCTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTT130.10312549579565286No Hit
GGGCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCC130.10312549579565286No Hit
GGGGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCAG130.10312549579565286No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130.10312549579565286No Hit
GGGACTCCCGAGCTCTACTGACTCCCAACAGAGCGCCCAAGAAGAAAATG130.10312549579565286No Hit
GGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCT130.10312549579565286No Hit
GGGGATTCTCTTTTCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCT130.10312549579565286No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)