FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12606
Sequences flagged as poor quality0
Sequence length36-200
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACATCCTGTCCGGCACTGCCCGCTTCTCCTCCAGGAGGTCCTTCTGGC670.5314929398699032No Hit
CACGTTCTCCTCCTGGTTATGGAAGTATCTGTCCAGGAACCGCACCCGCT550.4363001745200698No Hit
GGCTCAACTCGCCGCTGCACTGTGAAGCTCTCCACAACCCCGTAGTTGTG500.39663652229097257No Hit
CTCGTCTTCCAGGATGTCCTTCTGGCTGTTCCAGTACTCGGCATCAGGCC490.3887037918451531No Hit
GCATACCCTGTCCGGCACTGCCCGCTTCTCCTCCAGGAGGTCCTTCTGGC470.3728383309535142No Hit
GGCTCAACTCGCCGCTGCACTGTGAAGCTCTCACCAACCCCGTAGTTGTG410.32524194827859754No Hit
CTCCACAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCCCGCT390.30937648738695855No Hit
GTGTCTCCCGGTCCCAATACTCCGGCCCCTCCTGCTCCACCCACGGCGCC310.24591464382040296No Hit
AGTCCTCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACG300.23798191337458352No Hit
GGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCA270.21418372203712518No Hit
GGTCTGGTGTCCCCAGCCAAAGCCAGTGGGGAGCTCAGCACCATCAGTGT260.20625099159130572No Hit
GTAATTGTCCACCTGGCCCCGCTTCTGCTCCAGGAGGTCCTTCTGGCTGT250.19831826114548629No Hit
CTCCACAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCCCGCC240.19038553069966682No Hit
CTGCACATCCTGTCCGGCACTGCCCGCTTCTCCTCCAGGAGGTCCTTCTG210.1665873393622085No Hit
GAGACAGCGTGGTGAGTCATATGCGTTTTGGGGGCGTCCGTGCGCTGCAG210.1665873393622085No Hit
TGGAAGTATCTGTCCAGGAACCGCACCCGCTCCGTCCCATTGAAGAAATG200.15865460891638902No Hit
CCTCCTGGTTATGGAAGTGTCTCTCCAGGAACCGCACCCGCTCCGTCCCA190.15072187847056956No Hit
GGGCCCGTTGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA180.14278914802475012No Hit
GCAGACCCTGTCCGGCACTGCCCGCTTCTCCTCCAGGAGGTCCTTCTGGC180.14278914802475012No Hit
CTCACCAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCCCGCC170.1348564175789307No Hit
TAGATGCATCTTTCCAGCAACCGCACCCGCTCCGTCCCATTGAAGAAATG160.12692368713311122No Hit
GGGTCTGGTCCTCCCCATCCCGCTGCCAGGTCAGTGTGATCTCCGCAGGG160.12692368713311122No Hit
CGCGGCTTCCAGGATGTCCTTCTGGCTGTTCCAGTACTCGGCATCAGGCC160.12692368713311122No Hit
CCACCGCGGCCCGCCTCTGCTCCAGGAGGTCCTTCTGGCTGTTCCAGTAC160.12692368713311122No Hit
CCTTAGGCTCAACTCGCCGCTGCACTGTGAAGCTCTCACCAACCCCGTAG150.11899095668729176No Hit
TTCCAGCAACCGCACCCGCTCCGTCCCATTGAAGAAATGACATTCAAACT150.11899095668729176No Hit
CCTCTTGGTTATAGATGCATCTTTCCAGCAACCGCACCCGCTCCGTCCCA150.11899095668729176No Hit
GTACTCCCCCACGTCGCTGTCGAAGCGCACGGACTCCTCTTGGTTATAGA140.11105822624147231No Hit
AGTCCTCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGTACTCCCCCACG140.11105822624147231No Hit
CACGGTCTCCTCCTGGTTATGGAAGTATCTGTCCAGGAACCGCACCCGCT130.10312549579565286No Hit
CTCACCAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCCCGCT130.10312549579565286No Hit
GTGTCTCCCGGTCCCAATATTCCGGCCCCTCCTGCTCTATCCACGGCGCC130.10312549579565286No Hit
CTCGTCTTCCAGGAGGTCCTTCTGGCTGTTCCAGTACTCGGCATCAGGCC130.10312549579565286No Hit
GCACTGTGAAGCTCTCCACAACCCCGTAGTTGTGTCTGCAGTAGGTGTCC130.10312549579565286No Hit
GAGTATAGTTGGAGCGCTTTGTCATGATTTCCAGGTTGGCTTTGTCCACA130.10312549579565286No Hit
GGCTTGGGAGGCTTGGGAAGCTTCATGCGCAGGTTCTCCAGCTGCAGGTT130.10312549579565286No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)