FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12126
Sequences flagged as poor quality0
Sequence length36-200
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGGTTATAGAAGTATCTGTCCAGGAACCGCACCCGCTCCGTCCCA710.5855187201055583No Hit
GCGTCAGGCCGCCCCAGCTCCGTCACCGCCCGGTACTCCCCCACGTCGCT600.49480455220188024No Hit
GCGCTAGGCCGCCCCAGCTCCGTCACCGCCCGGTACTCCCCCACGTCGCT530.43707735444499424No Hit
GCACCGTGAAGCTCTCACCAACCCCGTAGTTGTGTCTGCAGTAGGTGTCC420.34636318654131615No Hit
CTCCACAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCCCGCG410.33811644400461816No Hit
GCCACCGGGGTGCTCAGCATCGCCAGCATCAAGGTCACAGTTGCTACCCG280.2309087910275441No Hit
CACGTACTCCTCTTGGTTATAGAAGTATCTGTCCAGGAACCGCACCCGCT230.1896750783440541No Hit
GGCTCAACTCGCCGCTGCACCGTGAAGCTCTCACCAACCCCGTAGTTGTG230.1896750783440541No Hit
GGTACAGGAAGTAGGCGGTGGTGGCCTGGCCAGCGAGGAGCAGAGTCACC220.1814283358073561No Hit
CAAGTCCTCCTCTTGGTTATAGATGTCTCTGTGCAGGAACCGCACCCGCT220.1814283358073561No Hit
CCGCCTGGTTATAGAAGTATCTGTCCAGGAACCGCACCCGCTCCGTCCCA210.17318159327065807No Hit
GGCTCAACTCGCCGCTGCACTGTGAAGCTCTCACCAACCCCGTAGTTGTG200.16493485073396008No Hit
GGCTTGGGAGGCTTGGGAAGCTTCATGCGCAGGTTCTCCAGCTGCAGGTT200.16493485073396008No Hit
GGTCTGGTGTCCCCAGCCAAAGCCAGTGGGGAGCTCAGCACCATCAGTGT190.1566881081972621No Hit
CGCCCGATACACCCCCACGTCGCTGTCGAAGCGCGCGTACTCCTCTCGGT180.14844136566056407No Hit
GAACATCTCATCTTCATCAAATTCAAACATAAACTCCCCTGTTGGTCTAT180.14844136566056407No Hit
CCTCTTGGTTATAGAAGTATCTGTCCAGGAACCGCACCCGCTCCGTCCCA180.14844136566056407No Hit
CTCACCAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCCCGCG160.13194788058716805No Hit
CTGTTCATACCCGCGGAGGAAGCGCCTGTCGGGCCCCAGCTCGCAGCCAT160.13194788058716805No Hit
CACCCCGCACGCGCTCGGTCCCGTTGGTGAAGTAGCACATGCCCTTAAAC160.13194788058716805No Hit
GCGCTAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGTCGCT150.12370113805047006No Hit
CTCCACAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCGCGCC150.12370113805047006No Hit
GCGTCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGTCGCT150.12370113805047006No Hit
GCCACCGGGGTGCTCAGCATCGCCAGCATCAAGGTGACAGTTGCTACCCG150.12370113805047006No Hit
GCGCGAGGCCGCCCCAGCTCCGTCACCGCCCGGTACTCCCCCACGTCGCT140.11545439551377205No Hit
GCTCCAGGATGTCCTTCTGGCTGTTCCAGTACTCAGCGTCAGGCCGCCCC140.11545439551377205No Hit
GCTCGTAGTTGTGTCTGCATACCCTGTCCGGCACTGCCCGCTTCTCCTCC140.11545439551377205No Hit
GGTCATACCCGCGGAGGAGGCGCCCGTCGGGCCCCAGGTCGCAGCCGTAC130.10720765297707406No Hit
CCCGTAGAGTACTCCAAGAAACGTGGTCTGGTGTCCCCAGCCAAAGCCAG130.10720765297707406No Hit
ACTCTGACCCCTCCTGCTCCATCCACGGCGCCCGCGGCACCATCCTCGGA130.10720765297707406No Hit
GAGACAGCGTGGTGAGTCATATGCGTTTTGGGGGCGTCCGTGCGCTGCAG130.10720765297707406No Hit
GATACACAGTCACCTTAGGTTGGACTCGCCGCTGCACTGTGAAGCTCTCC130.10720765297707406No Hit
CTGTCCAGGAACCGCACCCGCTCCGTCCCATTGAAGAAATAACACTCACC130.10720765297707406No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)