FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17985
Sequences flagged as poor quality0
Sequence length36-200
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGGTTATAGAAGTATCTGTCCAGGAACCGCACCCGCTCCGTCCCA700.3892132332499305No Hit
CAAGTCCTCCTCTTGGTTATAGATGTCTCTGTGCAGGAACCGCACCCGCT680.3780928551570753No Hit
GGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCA660.3669724770642202No Hit
GGGCCCGTTGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA540.3002502085070892No Hit
CAGCTTTGCCATGTAGGAACCTCCAGGGAGCTTCAGACACACCATGCTGG410.22796775090353072No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC390.21684737281067556No Hit
CTCCACAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCCCGCG340.18904642757853765No Hit
GGCTCAACTCGCCGCTGCACTGTGAAGCTCTCCACAACCCCGTAGTTGTG320.17792604948568252No Hit
GATACACAGTCACCTTAGGCTCAACTCGCCGCTGCACTGTGAAGCTCTCA310.17236586043925495No Hit
CGGCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGTACTCCCCCACGTCG300.16680567139282734No Hit
TAGATGCATCTTTCCAGCAACCGCACCCGCTCCGTCCCATTGAAGAAATG300.16680567139282734No Hit
GGTTGGACTCGCCGCTGCACTGTGAAGCTCTCCACAACCCCGTAGTTGTG280.15568529329997222No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA260.14456491520711703No Hit
GGTACAGGAAGTAGGCGGTGGTGGCCTGGCCAGCGAGGAGCAGAGTCACC250.13900472616068948No Hit
CCTCTTGGTTATAGATGCATCTTTCCAGCAACCGCACCCGCTCCGTCCCA250.13900472616068948No Hit
CCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGT250.13900472616068948No Hit
CCTGTCTTCCAGGAAGTCCTTCTGGCTGTTCCAGTACTCAGCGTCAGGCC250.13900472616068948No Hit
GGCTCAACTCGCCGCTGCACTGTGAAGCTCTCACCAACCCCGTAGTTGTG230.1278843480678343No Hit
CACCCCGCACGCGCTCCGTCCCGTTGGTGAAGTAGCACAGGCCCTTAAAC230.1278843480678343No Hit
CCGCCTGGTTATAGAAGTATCTGTCCAGGAACCGCACCCGCTCCGTCCCA220.12232415902140673No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCT210.11676396997497916No Hit
GCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACC200.11120378092855157No Hit
GGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGTA190.105643591882124No Hit
CTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCC190.105643591882124No Hit
CTCACCAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCCCGCC180.1000834028356964No Hit
CTGTTCATACCCGCGGAGGAAGCGCCCGTCGGGCCCCAGCTCGCAGCCAT180.1000834028356964No Hit
GAGACAGCGTGGTGAGTCATATGCGTTTTGGGGGCGTCCGTGCGCTGCAG180.1000834028356964No Hit
CAAGGCCTCCTCTTGGTTATAGATGTCTCTGTGCAGGAACCGCACCCGCT180.1000834028356964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)