FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17103
Sequences flagged as poor quality0
Sequence length36-200
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCCTCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACG470.2748055896626323No Hit
CTCGTCTTCCAGGATGTCCTTCTGGCTGTTCCAGTACTCGGCATCAGGCC420.24557095246447994No Hit
CTCCTCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGT340.19879553294743615No Hit
CACGTTCTCCTCCTGGTTATGGAAGTATCTGTCCAGGTACCGCACCCGCT340.19879553294743615No Hit
GTAGTTGTCCACCCGGCCCCGCTTCTGCTCCAGGAGGTCCTTCTGGCTGT300.17540782318891424No Hit
GACTTACGCAGCTCCAAGAAACGTGGTCGGGTGTCCCCAGCGAAAGCCAG280.1637139683096533No Hit
GTAATTGTCCACCCGGCCCCGCTTCTGCTCCAGGAGGTCCTTCTGGCTGT250.14617318599076187No Hit
GGTACAGGAAGTAGGCGGTGGTGGCCTGGCCAGCGAGGAGCAGAGTCACC240.1403262585511314No Hit
GGTTATGGTGCTGCAGGGGCTGGGTCTTTGAAGGATACACAGTCACCTTA230.1344793311115009No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC220.12863240367187043No Hit
CCTTAGGATGGACTCGCCGCTGCACTGTGAAGCTCTCCACAACCCCGTAG210.12278547623223997No Hit
GGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCA210.12278547623223997No Hit
GCCACTGGGGTGCTCAGCATCGACAGCATCAAGGTCACAGTTGCTGCCCG210.12278547623223997No Hit
GCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGTCGCT200.1169385487926095No Hit
CCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGTCGCTGTCGAAGCGCA200.1169385487926095No Hit
CACGGTCTCCTCCTGGTTATGGAAGTATCTGTCCAGGTACCGCACCCGCT180.10524469391334855No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)