FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12917
Sequences flagged as poor quality0
Sequence length36-90
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT2752.1289773167144075No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCC2441.888983510102965No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC2241.7341487961600992No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTC1661.2851281257257878No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTG1561.2077107687543547No Hit
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC1561.2077107687543547No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCC1551.1999690330572113No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA1381.0683595262057752No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCC1381.0683595262057752No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1341.037392583417202No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA1290.9986839049314857No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG1270.9832004335371991No Hit
GGGGAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCT1150.8902996051714793No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTG1140.8825578694743361No Hit
GGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCAG990.7664318340171866No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC920.7122396841371835No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTC920.7122396841371835No Hit
GGGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACT910.7044979484400402No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTC870.6735310056514671No Hit
GGGGAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCT800.619338855771464No Hit
GGGGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTGT750.5806301772857475No Hit
GGGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA720.5574049701943176No Hit
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC690.5341797631028876No Hit
GGGGGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCT650.5032128203143145No Hit
GGGGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCTC640.4954710846171712No Hit
GGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA630.4877293489200279No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT590.45676240613145463No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG540.41805372764573817No Hit
GGGGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGT510.3948285205543083No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG490.37934504916002165No Hit
GGGGGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTG480.3716033134628784No Hit
GGGATAATTTGGAATGTGGGTGGAGGGGGATCATAGTTCTCCCTGAGTGA460.35611984206859176No Hit
GGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTG430.3328946349771619No Hit
GGGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCC410.3174111635828753No Hit
GGGCAAGGATTACATCGCCCTGAACGAGGATCTGCGCTCCTGGACCGCCG380.2941859564914454No Hit
GGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTGC380.2941859564914454No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTG380.2941859564914454No Hit
GGGCGCCTCCTCCGCGGGTATAACCAGTTCGCCTACGACGGCAAGGATTA370.2864442207943021No Hit
GGGGATAATTTGGAATGTGGGTGGAGGGGGATCATAGTTCTCCCTGAGTG360.2787024850971588No Hit
GGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTG360.2787024850971588No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTC340.26321901370287215No Hit
GGGATCATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGG340.26321901370287215No Hit
GGGGAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCT330.2554772780057289No Hit
GGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC320.2477355423085856No Hit
GGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTC320.2477355423085856No Hit
GGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACT310.2399938066114423No Hit
GGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAG300.23225207091429897No Hit
GGGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTT280.2167685995200124No Hit
GGGGGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGTCCTGTTCT270.20902686382286909No Hit
GGGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCT270.20902686382286909No Hit
GGGGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTGT260.2012851281257258No Hit
GGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG250.19354339242858248No Hit
GGGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTG240.1858016567314392No Hit
GGGGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGTCCTGTTCTC240.1858016567314392No Hit
GGGATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTCGTCTCA230.17805992103429588No Hit
GGGGGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTG230.17805992103429588No Hit
GGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG230.17805992103429588No Hit
GGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA220.17031818533715257No Hit
GGGGGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTGTCCTGTTCT210.16257644964000928No Hit
GGGGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTGTCCTGTTCTC200.154834713942866No Hit
GGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAACA200.154834713942866No Hit
GGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG190.1470929782457227No Hit
GGGGATCATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTG190.1470929782457227No Hit
GGGCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGC190.1470929782457227No Hit
GGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGC190.1470929782457227No Hit
GGGCCCCATAATTTGGAATGTGGGTGGAGGGGGATCATAGTTCTCCCTGA180.1393512425485794No Hit
GGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC180.1393512425485794No Hit
GGGGAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTT180.1393512425485794No Hit
GGGCATCAGGTCCGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC170.13160950685143608No Hit
GGGCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGC170.13160950685143608No Hit
GGGGCCCCATAATTTGGAATGTGGGTGGAGGGGGATCATAGTTCTCCCTG170.13160950685143608No Hit
GGGAGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCT170.13160950685143608No Hit
GGGGCTCTGGCCCCTGGTCCTGTCCTGTTCTCCAGCATGGTGTGTCTGAG160.1238677711542928No Hit
GGGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCT160.1238677711542928No Hit
GGGCCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAG160.1238677711542928No Hit
GGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAA160.1238677711542928No Hit
GGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCT150.11612603545714949No Hit
GGGGTCCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGA150.11612603545714949No Hit
GGGAGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGT150.11612603545714949No Hit
GGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT150.11612603545714949No Hit
GGGGAGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCC150.11612603545714949No Hit
GGGGGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTG140.1083842997600062No Hit
GGGCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTGTGCTCACAGTCA140.1083842997600062No Hit
GGGTCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTT140.1083842997600062No Hit
GGGGAGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCG140.1083842997600062No Hit
GGGAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGTCCTCCTGCTGC140.1083842997600062No Hit
GGGATATAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGT140.1083842997600062No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA140.1083842997600062No Hit
GGGAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTC140.1083842997600062No Hit
GGGCCCATAATTTGGAATGTGGGTGGAGGGGGATCATAGTTCTCCCTGAG140.1083842997600062No Hit
GGGCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTCGTCT140.1083842997600062No Hit
GGGGGTCCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAG130.1006425640628629No Hit
GGGGGCTCTGGCCCCTGGTCCTGTCCTGTTCTCCAGCATGGTGTGTCTGA130.1006425640628629No Hit
GGGGATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTCGTCTC130.1006425640628629No Hit
GGGGATCAGGTCCGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC130.1006425640628629No Hit
GGGACTCGGCCTCAGTTCCTCATCACTGTTCCTGTGCTCACAGTCATCAA130.1006425640628629No Hit
GGGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGAC130.1006425640628629No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)