FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19521
Sequences flagged as poor quality0
Sequence length36-200
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGT1290.6608268019056401No Hit
TTATAGAAGTATCTGTCCAGGAACCGCACCCGCTCCGTCCCATTGAAGAA1080.5532503457814661No Hit
CACGTTCTCCTCCTGGTTATGGAAGTATCTGTCCAGGAACCGCACCCGCT920.4712873315916193No Hit
GGCTCAACTCGCCGCTGCACTGTGAAGCTCTCACCAACCCCGTAGTTGTG600.3073613032119256No Hit
GGCTCAACTCGCCGCTGCACTGTGAAGCTCTCCACAACCCCGTAGTTGTG430.22027560063521334No Hit
GGTACAGGAAGTAGGCGGTGGTGGCCTGGCCAGCGAGGAGCAGAGTCACC410.2100302238614825No Hit
CTCCACAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCCCGCG410.2100302238614825No Hit
ACTCCTCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACG410.2100302238614825No Hit
CGGCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGTCG400.2049075354746171No Hit
CTCCACAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCCCGCT370.1895394703140208No Hit
GTAATTGTCCACCTGGCCCCGCTTCTGCTCCAGGAGGTCCTTCTGGCTGT370.1895394703140208No Hit
GAGACAGCGTGGTGAGTCATATGCGTTTTGGGGGCGTCCGTGCGCTGCAG360.18441678192715535No Hit
GCACTGTGAAGCTCTCCACAACCCCGTAGTTGTGTCTGCAGTAGGTGTCC340.1741714051534245No Hit
GGTCTGGTGTCCCCAGCCAAAGCCAGTGGGGAGCTCAGCACCATCAGTGT330.16904871676655908No Hit
GATACACAGTCACCTTAGGTTGGACTCGCCGCTGCACTGTGAAGCTCTCC330.16904871676655908No Hit
CTCGTCTTCCAGGATGTCCTTCTGGCTGTTCCAGTACTCGGCATCAGGCC320.16392602837969367No Hit
CTCCACAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCGCGCC310.15880333999282822No Hit
CTGTCCAGGAACCGCACCCGCTCCGTCCCGTTGAAGAAATGACACTCATA290.14855796321909737No Hit
GAGACAGCGTGGTGAGTCATATGCGTCTTGGGGGCGTCCGTGCGCTGCAG290.14855796321909737No Hit
CTCACCAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCGCGCC290.14855796321909737No Hit
GATACACAGTCACCTTAGGCTCAACTCGCCGCTGCACTGTGAAGCTCTCA280.14343527483223195No Hit
GATACACAGTCACCTTAGGATGGACTCGCCGCTGCACTGTGAAGCTCTCC280.14343527483223195No Hit
GTGTCTCCCGGTCCCAATACTCCGGCCCCTCCTGCTCCACCCACGGCGCC260.1331898980585011No Hit
CGCCGCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGT260.1331898980585011No Hit
GTGTCTCCCGGTCCCAATACTCCGGCCCCTCCTGCTCTATCCACGGCGCC250.12806720967163568No Hit
GCTCCACCCACGGCGCCCGCGGCTCCCCTCTCGGACTCGCGGCGTCGCTG250.12806720967163568No Hit
CCTCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGTCG240.12294452128477024No Hit
GTTATAGAAGTATCTGTCCAGGAACCGCACCCGCTCCGTCCCATTGAAGA230.11782183289790482No Hit
GGTCATACCCGCGGAGGAGGCGCCCGTCGGGCCCCACGTCGCAGCCATAC220.1126991445110394No Hit
CGCCGCTTCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGT210.10757645612417396No Hit
CACGTTCTCCTCCTGGTTATAGAAGTATCTGTCCAGGAACCGCACCCGCT210.10757645612417396No Hit
ACTCAGCGTCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACG210.10757645612417396No Hit
CACGGTCTCCTCCTGGTTATGGAAGTATCTGTCCAGGAACCGCACCCGCT210.10757645612417396No Hit
ACTCTGACCCCTCCTGCTCCATCCACGGCGCCCGCGGCACCATCCTCGGA210.10757645612417396No Hit
CTGTCCAGGAACCGCACCCGCTCCGTCCCATTGAAGAAATGACACTCCCT210.10757645612417396No Hit
GTACACCCCCACGTCGCTGTCGAAGCGCGCGTACTCCTCTCGGTTATAGA210.10757645612417396No Hit
CCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGTCGCTGTCGAAGCGCA200.10245376773730855No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)