FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19860
Sequences flagged as poor quality0
Sequence length36-200
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGT970.48841893252769386No Hit
CACGTTCTCCTCCTGGTTATGGAAGTATCTGTCCAGGTACCGCACCCGCT820.41289023162134947No Hit
GGTACACGTAATTCTCTGGAGTGGCCCTGCCCTGGACCACAGATGTGAGC640.32225579053373615No Hit
GAACATCTCATCTTCATCAAATTCAAACATAAACTCCCCTGTTGGTCTAT590.297079556898288No Hit
TGGAAGTATCTGTCCAGGTACCGCACCCGCTCCGTCCCATTGAAGAAATG560.28197381671701915No Hit
CTCCACAACCCCGTAGTTGTGTCTGCAGTAGTTGTCCACCCGGCCCCGCT510.256797583081571No Hit
GCACATCCTGTCCGGCACTGCCCGCTTCTCCTCCAGGATGTCCTTCTGGC440.2215508559919436No Hit
GGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCA440.2215508559919436No Hit
CGCGTACTCCTCCCGGTTGTAGATGTATCTCTCCAGGAAGCGCTGTGTCC420.21148036253776434No Hit
GTCACGGCCTCGTCCAGCTCGTAGTTGTGTCTGCATACCCTGTCCGGCAC380.19133937562940584No Hit
GGTCTGGTGTCCCCAGCCAAAGCCAGTGGGGAGCTCAGCACCATCAGTGT350.17623363544813697No Hit
CCTCCTGGTTATGGAAGTATCTGTCCAGGTACCGCACCCGCTCCGTCCCA350.17623363544813697No Hit
GGCTCAACTCGCCGCTGCACTGTGAAGCTCTCACCAACCCCGTAGTTGTG350.17623363544813697No Hit
GCATACCCTGTCCGGCACTGCCCGCTTCTCCTCCAGGATGTCCTTCTGGC330.1661631419939577No Hit
CACGGTCTCCTCCTGGTTATGGAAGTATCTGTCCAGGTACCGCACCCGCT320.16112789526686808No Hit
GGTTATGGTGCTGCAGGGGCTGGGTCTTTGAAGGATACACAGTCACCTTA290.14602215508559918No Hit
CACGAACTCCTCCCGGTTGTAGATGTATCTCTCCAGGAAGCGCTGTGTCC290.14602215508559918No Hit
CCACCGCGGCCCGCCTCTGCTCCAGGAGGTCCTTCTGGCTGTTCCAGTAC290.14602215508559918No Hit
GGGCCCGTTGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA290.14602215508559918No Hit
CTGCACATCCTGTCCGGCACTGCCCGCTTCTCCTCCAGGATGTCCTTCTG260.1309164149043303No Hit
GGCTCAACTCGCCGCTGCACTGTGAAGCTCTCCACAACCCCGTAGTTGTG250.12588116817724068No Hit
GTCACGGCCTCGTCCAGCTCGTAGTTGTGTCTGCACATCCTGTCCGGCAC250.12588116817724068No Hit
GTAGTTGTCCACCCGGCCCCGCTTCTGCTCCAGGAGGTCCTTCTGGCTGT240.12084592145015105No Hit
CGCCGCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGT230.11581067472306143No Hit
GGGACACGTAATTCTCTGGAGTGGCCCTGCCCTGGACCACAGATGTGAGC230.11581067472306143No Hit
ACTCCTCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACG230.11581067472306143No Hit
CGCCGCTTCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGT220.1107754279959718No Hit
GGTACAGGAAGTAGGCGGTGGTGGCCTGGCCAGCGAGGAGCAGAGTCACC210.10574018126888217No Hit
GAACTGCTCATCTTCATCAAATTCAAACATAAACTCCCCTGTTGGTCTAT200.10070493454179255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)