FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004963830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202253
Sequences flagged as poor quality0
Sequence length36-90
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC26221.2963960979565199No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG24731.2227259917034605No Hit
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC24051.1891047351584403No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT20461.011604277810465No Hit
GGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT18510.9151903803651862No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA14910.7371954927739021No Hit
GGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG13290.6570977933578241No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG12600.6229821065694947No Hit
GGGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAAC12560.6210043855962581No Hit
GGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA10770.5325013720439251No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT9930.49096923160595884No Hit
GGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAG9490.4692143009003575No Hit
GGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTGT8970.44350392824828305No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG8870.43855962581519187No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA8600.42521000924584557No Hit
GGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG8020.3965330551339164No Hit
GGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAA7720.38170014783464273No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG7500.370822682481842No Hit
GGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC7470.36933939175191466No Hit
GGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTT7250.358461926399114No Hit
GGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCA6520.322368518637548No Hit
GGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG5600.2768809362531087No Hit
GGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGA5520.27292549430663576No Hit
GGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC5380.266003470900308No Hit
GGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGC5310.2625424591971442No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT5300.26204802895383505No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC5220.2580925870073621No Hit
GGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACG4800.2373265167883789No Hit
GGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGTT4520.22348246997572346No Hit
GGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCTC4310.2130994348662319No Hit
GGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTGGTGC4190.2071662719465224No Hit
GGGCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGCG4160.20568298121659503No Hit
GGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAG4020.19876095781026734No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTG3980.19678323683703083No Hit
GGGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAA3810.18837792270077575No Hit
GGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATG3800.18788349245746663No Hit
GGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACAGTGC3590.17750045734797507No Hit
GGGCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTCTGG3460.17107286418495649No Hit
GGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGT3430.1695895734550291No Hit
GGGCCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCTA3280.16217311980539226No Hit
GGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT3250.1606898290754649No Hit
GGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGGCCTC3190.15772324761561016No Hit
GGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGTGCATA3150.15574552664237368No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3080.1522845149392098No Hit
GGGCTTTTTCGTCTGGGCTGCCAACATGCCATCCAGACTGAGGAAGACCC3030.1498123637226642No Hit
GGGCCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGC2990.1478346427494277No Hit
GGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCA2970.14684578226280945No Hit
GGGCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAG2890.14289034031633646No Hit
GGGCTTTTCCTGTGGCAGCAGCCGGGCTGAGAGGAGCGTGGCTGTCTCCT2830.13992375885648173No Hit
GGGATGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT2660.13151844472022664No Hit
GGGGCTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGG2640.13052958423360841No Hit
GGGCTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCAT2640.13052958423360841No Hit
GGGCTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGC2630.13003515399029927No Hit
GGGATTGCAGACTGCGGAGTCAGACGGCGCTATGTACGCCCTCTTCCTCC2620.12954072374699016No Hit
GGGAGCCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTC2580.12756300277375368No Hit
GGGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTGGTG2570.12706857253044454No Hit
GGGCTCTTTCCCATCTTGCAAGATGGCGGGTGAAAAAGTTGAGAAGCCAG2570.12706857253044454No Hit
GGGGCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGC2560.12657414228713543No Hit
GGGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCT2540.12558528180051717No Hit
GGGGCCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCT2400.11866325839418945No Hit
GGGCTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTT2400.11866325839418945No Hit
GGGCTTTTCACAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCCT2380.1176743979075712No Hit
GGGCCTTTCGTTGCCTGATCGCCGCCATCATGGGTCGCATGCATGCTCCC2350.11619110717764383No Hit
GGGGCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGA2310.11421338620440735No Hit
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC2250.11124680474455262No Hit
GGGCCCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCT2230.11025794425793436No Hit
GGGGCCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAG2220.10976351401462525No Hit
GGGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGTGCAT2140.10580807206815226No Hit
GGGGCCCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGC2130.10531364182484314No Hit
GGGCTTTCCTTTCAGCGGAGCGCGGCGGCAAGATGGCAGTGCAAATATCC2100.10383035109491577No Hit
GGGGCTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCA2080.10284149060829752No Hit
GGGGCTCTTTCCCATCTTGCAAGATGGCGGGTGAAAAAGTTGAGAAGCCA2080.10284149060829752No Hit
GGGGCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTCTG2070.1023470603649884No Hit
GGGCTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGGTG2050.10135819987837015No Hit
GGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGGCCT2030.10036933939175192No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)