FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41443
Sequences flagged as poor quality0
Sequence length36-90
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCC8482.0461839152571No Hit
GGGGATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTC6051.4598364018048886No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT5561.3416017180223438No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC4851.1702820741741669No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA4110.9917235721352219No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCC3920.9458774702603576No Hit
GGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCAG3750.9048572738460053No Hit
GGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTG3520.8493593610501171No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTG3070.7407764881885964No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTC3010.7262987718070604No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2940.7094081026952682No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCC2930.7069951499650122No Hit
GGGGGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCT2910.7021692445045001No Hit
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC2850.6876915281229641No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA2790.673213811741428No Hit
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC2770.668387906280916No Hit
GGGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA2550.6153029462152837No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT2500.6032381825640035No Hit
GGGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCT2490.6008252298337475No Hit
GGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAAA2490.6008252298337475No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG2440.5887604661824675No Hit
GGGGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCTC2370.5718697970706754No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTC2350.5670438916101633No Hit
GGGCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT2350.5670438916101633No Hit
GGGGAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCT2330.5622179861496513No Hit
GGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA2250.5429143643076032No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC2250.5429143643076032No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG1840.44398330236710665No Hit
GGGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACT1800.4343314914460826No Hit
GGGCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC1800.4343314914460826No Hit
GGGGGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCTTGTCCTCTTCT1660.4005501532224984No Hit
GGGGATAATTTGGAATGTGGGTGGAGGGGGATCATAGTTCTCCCTGAGTG1570.37883357865019424No Hit
GGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTG1540.3715947204594262No Hit
GGGATAATTTGGAATGTGGGTGGAGGGGGATCATAGTTCTCCCTGAGTGA1520.36676881499891417No Hit
GGGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTT1520.36676881499891417No Hit
GGGGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGT1510.3643558622686581No Hit
GGGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAA1510.3643558622686581No Hit
GGGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCC1500.36194290953840214No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG1440.347465193156866No Hit
GGGCCATCAGGTCCGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT1400.337813382235842No Hit
GGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA1330.3209227131240499No Hit
GGGCTCACAATTGCTCTACAGCTCAGAACAGCAACTGCTGAGGCTGCCTT1320.31850976039379386No Hit
GGGGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCTTGTCCTCTTCTC1310.31609680766353787No Hit
GGGGCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTC1300.3136838549332819No Hit
GGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTC1290.31127090220302583No Hit
GGGATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCA1260.3040320440122578No Hit
GGGAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTC1250.30161909128200176No Hit
GGGAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCTG1170.28231546943995367No Hit
GGGATAGTTCTCCCTGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCT1150.27748956397944163No Hit
GGGCATCAGGTCCGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC1140.2750766112491856No Hit
GGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG1130.2726636585189296No Hit
GGGGATAGTTCTCCCTGATTGAGACTTGCCTGCTGCTGTGACCACTGGTC1100.26542480032816157No Hit
GGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC1040.2509470839466255No Hit
GGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC1000.24129527302560144No Hit
GGGGAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTT970.2340564148348334No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTG960.2316434621045774No Hit
GGGATCAGGTCCGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCA930.22440460391380934No Hit
GGGATCATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGG900.2171657457230413No Hit
GGGGATCATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTG840.20268802934150518No Hit
GGGCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGC840.20268802934150518No Hit
GGGGAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCT820.19786212388099317No Hit
GGGGCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT800.19303621842048116No Hit
GGGGAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCT800.19303621842048116No Hit
GGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG780.18821031295996912No Hit
GGGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTG760.18338440749945709No Hit
GGGGCCATCAGGTCCGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTC760.18338440749945709No Hit
GGGCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTGTGCTCACAGTCA730.17614554930868903No Hit
GGGGGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCT720.173732596578433No Hit
GGGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCT710.17131964384817702No Hit
GGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGG710.17131964384817702No Hit
GGGACTCGGCCTCAGTTCCTCATCACTGTTCCTGTGCTCACAGTCATCAA710.17131964384817702No Hit
GGGGGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTG700.168906691117921No Hit
GGGCCCCATAATTTGGAATGTGGGTGGAGGGGGATCATAGTTCTCCCTGA700.168906691117921No Hit
GGGGGAGAGGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTG690.16649373838766499No Hit
GGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG690.16649373838766499No Hit
GGGGCTCACAATTGCTCTACAGCTCAGAACAGCAACTGCTGAGGCTGCCT680.16408078565740897No Hit
GGGGATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC670.16166783292715295No Hit
GGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTT670.16166783292715295No Hit
GGGGGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGTCCTGTTCT650.15684192746664094No Hit
GGGCTGGCTAACATTGCTATATTGAACAACAACTTGAATACCTTGATCCA630.1520160220061289No Hit
GGGGCATCAGGTCCGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT630.1520160220061289No Hit
GGGAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTACTTTTCCCTTC630.1520160220061289No Hit
GGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAG630.1520160220061289No Hit
GGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGC620.14960306927587289No Hit
GGGACTCCCGAGCTCTACTGACTCCCAAAAGAGCGCCCAAGAAGAAAATG620.14960306927587289No Hit
GGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT610.14719011654561687No Hit
GGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACT580.13995125835484884No Hit
GGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGACT580.13995125835484884No Hit
GGGGCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTG580.13995125835484884No Hit
GGGGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGTCCTGTTCTC570.1375383056245928No Hit
GGGACAGTTTCAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCAC560.1351253528943368No Hit
GGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGTT560.1351253528943368No Hit
GGGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGAC550.13271240016408078No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTC550.13271240016408078No Hit
GGGGGGAGAGGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCT530.12788649470356875No Hit
GGGGCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTGTGCTCACAGTC530.12788649470356875No Hit
GGGGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTGT520.12547354197331276No Hit
GGGTCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTC510.12306058924305673No Hit
GGGCTCACAATTACTCTACAGCTCAGAACACCAACTGCTGAGGCTGCCTT510.12306058924305673No Hit
GGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCTC500.12064763651280072No Hit
GGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAA500.12064763651280072No Hit
GGGATATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGT500.12064763651280072No Hit
GGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGT500.12064763651280072No Hit
GGGGAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTACTTTTCCCTT490.1182346837825447No Hit
GGGAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCAATT490.1182346837825447No Hit
GGGCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCC470.11340877832203267No Hit
GGGGACTCCCGAGCTCTACTGACTCCCAAAAGAGCGCCCAAGAAGAAAAT470.11340877832203267No Hit
GGGGCCCTTTCATCAGTCTTCCCGCGTCCGCCGATTCCTCCTCCTTGGTC470.11340877832203267No Hit
GGGGCCCCATAATTTGGAATGTGGGTGGAGGGGGATCATAGTTCTCCCTG460.11099582559177666No Hit
GGGGAGCTCTACTGACTCCCAAAAGAGCGCCCAAGAAGAAAATGGCCATA460.11099582559177666No Hit
GGGGACTCGGCCTCAGTTCCTCATCACTGTTCCTGTGCTCACAGTCATCA460.11099582559177666No Hit
GGGGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCTC450.10858287286152064No Hit
GGGGCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTC440.10616992013126464No Hit
GGGGATATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGG430.10375696740100862No Hit
GGGGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTT430.10375696740100862No Hit
GGGAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTCCA420.10134401467075259No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)