FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20358
Sequences flagged as poor quality0
Sequence length36-200
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGTTCTCCTCCTGGTTATGGAAGTATCTGTCCAGGAACCGCACCCGCT890.43717457510560953No Hit
GGCTCAACTCGCCGCTGCACTGTGAAGCTCTCACCAACCCCGTAGTTGTG830.4077021318400629No Hit
TTATAGAAGTATCTGTCCAGGAACCGCACCCGCTCCGTCCCATTGAAGAA800.39296591020728955No Hit
GTAATTGTCCACCTGGCCCCGCTTCTGCTCCAGGAGGTCCTTCTGGCTGT560.27507613714510265No Hit
CTCCACAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCCCGCG520.2554278416347382No Hit
GTCCTCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGT480.23577954612437368No Hit
GTCACGGCCTCGTCCAGCTCGTAGTTGTGTCTGCATACCCTGTCCGGCAC460.22595539836919148No Hit
GGTACAGGAAGTAGGCGGTGGTGGCCTGGCCAGCGAGGAGCAGAGTCACC400.19648295510364477No Hit
GGTCTGGTGTCCCCAGCCAAAGCCAGTGGGGAGCTCAGCACCATCAGTGT380.18665880734846252No Hit
GCACATCCTGTCCGGCACTGCCCGCTTCTCCTCCAGGATGTCCTTCTGGC380.18665880734846252No Hit
GGTCATACCCGCGGAGGAGGCGCCCGTCGGGCCCCACGTCGCAGCCATAC380.18665880734846252No Hit
GGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCA360.17683465959328026No Hit
GCACTGTGAAGCTCTCACCAACCCCGTAGTTGTGTCTGCAGTAGGTGTCC350.17192258571568916No Hit
CTCGTCTTCCAGGATGTCCTTCTGGCTGTTCCAGTACTCGGCATCAGGCC330.1620984379605069No Hit
CTGTCCAGGAACCGCACCCGCTCCGTCCCGTTGAAGAAATGACACTCATA310.15227429020532468No Hit
GTCACGGCCTCGTCCAGCTCGTAGTTGTGTCTGCACATCCTGTCCGGCAC310.15227429020532468No Hit
CGGCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGTCG300.14736221632773355No Hit
CTCCACAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCCCGCT300.14736221632773355No Hit
GCATACCCTGTCCGGCACTGCCCGCTTCTCCTCCAGGAGGTCCTTCTGGC300.14736221632773355No Hit
GTACCCCACCTCGTAGTTGTGTCTGCACACCGTGTCCAACTCCGCCCGGG280.13753806857255133No Hit
CACGTTCTCCTCCTGGTTATAGAAGTATCTGTCCAGGAACCGCACCCGCT260.1277139208173691No Hit
GTGTCTCCCGGTCCCAATATTCCGGCCCCTCCTGCTCTATCCACGGCGCC250.12280184693977797No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC240.11788977306218684No Hit
GCACATCCTGTCCGGCACTGCCCGCTTCTCCTCCAGGAGGTCCTTCTGGC240.11788977306218684No Hit
GCACTGTGAAGCTCTCCACAACCCCGTAGTTGTGTCTGCAGTAGGTGTCC240.11788977306218684No Hit
GGTTATAGAAGTATCTGTCCAGGAACCGCACCCGCTCCGTCCCATTGAAG240.11788977306218684No Hit
GCGCACGTTCTCCTCCTGGTTATGGAAGTATCTGTCCAGGAACCGCACCC230.11297769918459574No Hit
GGGCCCGTTGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA220.10806562530700461No Hit
CTCGTCTTCCAGGATGTCCTTCTGGCTGTTCCAGTACTCAGCGTCAGGCC220.10806562530700461No Hit
CCACCGCGGCCCGCGCCTGCTCCAGGATGTCCTTCTGGCTGTTCCAGTAC220.10806562530700461No Hit
GGCACGGCCTCGTCCAGCTCGTAGTTGTGTCTGCATACCCTGTCCGGCAC220.10806562530700461No Hit
CTGTTCATACCCGCGGAGGAAGCGCCTGTCGGGCCCCAGCTCGCAGCCAT210.10315355142941349No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)