FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90761
Sequences flagged as poor quality0
Sequence length36-90
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT13861.5270876257423343No Hit
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC13161.4499619880785801No Hit
GGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA8940.9850045724485187No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG8870.9772920086821433No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG7780.8571963728914401No Hit
GGGCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTCTGG5150.5674243342404777No Hit
GGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAG5110.563017154945406No Hit
GGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCA5030.5542027963552627No Hit
GGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGC4950.5453884377651195No Hit
GGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAA4740.5222507464659931No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG4590.5057238241094744No Hit
GGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC4420.4869933121054197No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA4090.4506340829210784No Hit
GGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG4040.4451251088022389No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC3990.4396161346833993No Hit
GGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC3970.43741254503586346No Hit
GGGGCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTCTG3670.40435870032282595No Hit
GGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG3430.37791562455239586No Hit
GGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT3380.37240665043355625No Hit
GGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTGT3210.3536761384295017No Hit
GGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATG3160.3481671643106621No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT3050.336047421249215No Hit
GGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCA2970.32723306265907165No Hit
GGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG2960.3261312678353037No Hit
GGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGA2720.2996881920648737No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT2680.295281012769802No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC2660.2930774231222662No Hit
GGGTGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT2390.26332896288053237No Hit
GGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT2350.25892178358546075No Hit
GGGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAAC2310.254514604290389No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA2280.2512092198190853No Hit
GGGAGCCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTC2170.2390894767576382No Hit
GGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTT2030.22366434922488737No Hit
GGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACG2020.22256255440111944No Hit
GGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGTT1950.21484999063474403No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1950.21484999063474403No Hit
GGGCCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGC1710.18840691486431396No Hit
GGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCTC1700.18730512004054606No Hit
GGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGT1680.18510153039301022No Hit
GGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAG1630.17959255627417062No Hit
GGGGAGCCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTT1620.1784907614504027No Hit
GGGATGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT1620.1784907614504027No Hit
GGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGAC1570.1729817873315631No Hit
GGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACA1440.1586584546225802No Hit
GGGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAA1300.14323332708982933No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTG1270.13992794261852556No Hit
GGGCTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTT1270.13992794261852556No Hit
GGGAGTGTGAAATCTTCAGAGAAGAATTTCTCTTTAGTTCTTTGCAAGAA1250.13772435297098976No Hit
GGGGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACC1240.13662255814722182No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG1220.134418968499686No Hit
GGGCTTTTCACAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCCT1200.13221537885215015No Hit
GGGCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGCG1190.13111358402838222No Hit
GGGCTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCAT1190.13111358402838222No Hit
GGGGACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC1180.13001178920461431No Hit
GGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTGGTGC1170.1289099943808464No Hit
GGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGGCCTC1150.12670640473331055No Hit
GGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGTGCATA1150.12670640473331055No Hit
GGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAAC1130.12450281508577474No Hit
GGGGCTCTTTCCCATCTTGCAAGATGGCGGGTGAAAAAGTTGAGAAGCCA1100.12119743061447098No Hit
GGGCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAA1090.12009563579070306No Hit
GGGCCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAA1090.12009563579070306No Hit
GGGCTCTTTCCCATCTTGCAAGATGGCGGGTGAAAAAGTTGAGAAGCCAG1070.11789204614316721No Hit
GGGCCTTTTCACAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC1050.11568845649563139No Hit
GGGGCCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAG1040.11458666167186346No Hit
GGGCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAG1000.11017948237679179No Hit
GGGGCTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCA980.10797589272925597No Hit
GGGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCT980.10797589272925597No Hit
GGGTAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC960.10577230308172011No Hit
GGGCTCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACC960.10577230308172011No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)