FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30311
Sequences flagged as poor quality0
Sequence length36-90
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT24938.224736894196827No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC24598.112566395038105No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA13714.523110421959025No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG11403.76101085414536No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCC4691.5472930619247138No Hit
GGGATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCC4601.5176008709709345No Hit
GGGGATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTC4021.326251195935469No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTC3331.0986110652898289No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA3231.065619742007852No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTG3101.022731021741282No Hit
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC2690.8874665962851769No Hit
GGGGAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCT2640.8709709346441885No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC2260.7456039061726766No Hit
GGGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACT2100.6928177889215136No Hit
GGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCCTCCTGCTGC1850.6103394807165715No Hit
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC1290.4255880703375012No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT1170.38599848239912904No Hit
GGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCAG1080.35630629144534987No Hit
GGGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCCTCCTGCTG990.32661410049157075No Hit
GGGGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTGT970.32001583583517534No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTC910.30022104186598925No Hit
GGGGGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCT870.2870245125531985No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCC840.2771271155686054No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGCC760.250734056943024No Hit
GGGGGGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCC750.2474349246148263No Hit
GGGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA750.2474349246148263No Hit
GGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACCC750.2474349246148263No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGC740.24413579228662863No Hit
GGGAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCTG610.20124707202005873No Hit
GGGGGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTG610.20124707202005873No Hit
GGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTG600.19794793969186106No Hit
GGGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACC600.19794793969186106No Hit
GGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA590.19464880736366336No Hit
GGGAGGGGCGGTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGT570.18805054270726798No Hit
GGGGAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCT560.1847514103790703No Hit
GGGAGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGT540.17815314572267493No Hit
GGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG530.17485401339447726No Hit
GGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTG530.17485401339447726No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG530.17485401339447726No Hit
GGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTC530.17485401339447726No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG520.17155488106627956No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCCCCATGGTGCTGT520.17155488106627956No Hit
GGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAACA500.1649566164098842No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC490.1616574840816865No Hit
GGGCCCTATAATTTGGAATGTGGGTGGAGAGGGGTCATAGTTCTCCCTGA490.1616574840816865No Hit
GGGCAAGGATTACATCGCCCTGAACGAGGACCTGCGCTCCTGGACCGCCG490.1616574840816865No Hit
GGGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAC490.1616574840816865No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGCACCCACCATGGTGCTGT480.15835835175348884No Hit
GGGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTT450.1484609547688958No Hit
GGGTTACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAG440.1451618224406981No Hit
GGGTACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA420.1385635577843027No Hit
GGGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTG420.1385635577843027No Hit
GGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCAAGCTGTGTTGACT400.13196529312790736No Hit
GGGCCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT400.13196529312790736No Hit
GGGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCC390.1286661607997097No Hit
GGGAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGTCCTCCTGCTGC390.1286661607997097No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG390.1286661607997097No Hit
GGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC390.1286661607997097No Hit
GGGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGAC390.1286661607997097No Hit
GGGCCCTTTCATCAGTCTTCCCGCGTCCGCCGATTCCTCCTCCTTGGTCG380.125367028471512No Hit
GGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGG380.125367028471512No Hit
GGGGGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCT380.125367028471512No Hit
GGGAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTC380.125367028471512No Hit
GGGCTCACAATTACTCTACAGCTCAGAACAGCAACTGCTGAGGCTGCCTT370.12206789614331431No Hit
GGGGCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTCGTC370.12206789614331431No Hit
GGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACT370.12206789614331431No Hit
GGGATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCA370.12206789614331431No Hit
GGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG350.11546963148691895No Hit
GGGCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTCGTCT350.11546963148691895No Hit
GGGGCCCTATAATTTGGAATGTGGGTGGAGAGGGGTCATAGTTCTCCCTG340.11217049915872125No Hit
GGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA340.11217049915872125No Hit
GGGATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTCGTCTCA330.10887136683052356No Hit
GGGGGAGAGGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTG330.10887136683052356No Hit
GGGGCCCTTTCATCAGTCTTCCCGCGTCCGCCGATTCCTCCTCCTTGGTC330.10887136683052356No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG330.10887136683052356No Hit
GGGACACTCGCTTCTGGAACGTCTGAGGTTATCAATAAGCTCCTAGTCCA320.10557223450232589No Hit
GGGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCT320.10557223450232589No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)