FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30311
Sequences flagged as poor quality0
Sequence length36-200
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCA2080.6862195242651182No Hit
GGGCCCGTTGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA1750.5773481574345947No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC1360.448681996634885No Hit
CACGTTCTCCTCCTGGTTATGGAAGTATCTGTCCAGGAACCGCACCCGCT910.30022104186598925No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA880.2903236448813962No Hit
CCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGT840.2771271155686054No Hit
GGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGTA830.27382798324040775No Hit
GCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACC820.2705288509122101No Hit
GGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAG640.21114446900465178No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCT630.2078453366764541No Hit
GCACACGGTGTCCACCTGGCCCCGCCTGTCCTCCAGGATGTCCTTCTGGC630.2078453366764541No Hit
GCTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTG570.18805054270726798No Hit
CTGCACACGGTGTCCACCTGGCCCCGCCTGTCCTCCAGGATGTCCTTCTG560.1847514103790703No Hit
CCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGC550.18145227805087263No Hit
CTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCC520.17155488106627956No Hit
GGTACAGGAAGTAGGCGGTGGTGGCCTGGCCAGCGAGGAGCAGAGTCACC500.1649566164098842No Hit
GCACTGTGAAGCTCTCCACAACCCCGTAGTTGTGTCTGCAGTAGGTGTCC490.1616574840816865No Hit
GCCACCGGGGTGCTCAGCATCGCCAGCATCAAGGTCACAGTTGCTACCCG470.15505921942529113No Hit
GTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAG440.1451618224406981No Hit
CCTCAGGATGGACTCGCCGCTGCACTGTGAAGCTCTCACCAACCCCGTAG440.1451618224406981No Hit
GCACTGTGAAGCTCTCACCAACCCCGTAGTTGTGTCTGCACACGGTGTCC440.1451618224406981No Hit
GCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGC420.1385635577843027No Hit
CCGGAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC420.1385635577843027No Hit
CTCCACAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCCCGCT400.13196529312790736No Hit
GGTCTGGTGTCCCCAGCCAAAGCCAGTGGGGAGCTCAGCACCATCAGTGT390.1286661607997097No Hit
GTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCAT370.12206789614331431No Hit
TCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCAG360.11876876381511663No Hit
GAGACAGCGTGGTGAGTCATATGCGTTTTGGGGGCGTCCGTGCGCTGCAG360.11876876381511663No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT350.11546963148691895No Hit
CTGCACACGGTGTCCACCGCGGCCCGCTCGTCTTCCAGGATGTCCTTCTG340.11217049915872125No Hit
CCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGTCGCTGTCGAAGCGCA340.11217049915872125No Hit
GCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCA340.11217049915872125No Hit
CGCCCGATACACCCCCACGTCGCTGTCGAAGCGTGCGTACTCCTCTCGGT340.11217049915872125No Hit
GGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTC330.10887136683052356No Hit
GCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTTAACGGTATTT330.10887136683052356No Hit
GGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGC330.10887136683052356No Hit
GCCACCGGGGTGCTCAGCATCGCCAGCATCAAGGTGACAGTTGCTACCCG330.10887136683052356No Hit
GCCCAGGGCCTCACCACCAACTTCATCCACGTTCACCTTGCCCCACAGGG320.10557223450232589No Hit
CCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCAC320.10557223450232589No Hit
GTGTCTCCCGGTCCCAATACTCCGGCCCCTCCTGCTCCACCCACGGCGCC310.1022731021741282No Hit
GCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCTTGTCACAGTGCA310.1022731021741282No Hit
CCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGC310.1022731021741282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)