FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13746
Sequences flagged as poor quality0
Sequence length36-90
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCC3122.2697512003491926No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCC2551.8550851156700132No Hit
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC2291.6659391823075804No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTG2191.5931907463989523No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA2071.5058926233085987No Hit
GGGGAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCT1971.433144187399971No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTC1921.3967699694456568No Hit
GGGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACT1881.3676705950822057No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTG1711.2439982540375383No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTC1581.1494252873563218No Hit
GGGGAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCT1501.0912265386294195No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC1240.9020806052669867No Hit
GGGGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTGT990.7202095154954169No Hit
GGGGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGT980.7129346719045541No Hit
GGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTG780.5674378000872982No Hit
GGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCAG740.5383384257238469No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT720.5237887385421214No Hit
GGGGGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTG640.46558998981521893No Hit
GGGGGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGTCCTGTTCT630.45831514622435615No Hit
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC610.4437654590426306No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCC590.42921577186090504No Hit
GGGCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT570.41466608467917937No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTC550.4001163974974538No Hit
GGGGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGTCCTGTTCTC540.39284155390659103No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG540.39284155390659103No Hit
GGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTGC540.39284155390659103No Hit
GGGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA520.3782918667248654No Hit
GGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAACA500.3637421795431398No Hit
GGGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCT490.35646733595227703No Hit
GGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTC480.3491924923614142No Hit
GGGGGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTG470.3419176487705514No Hit
GGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA460.33464280517968864No Hit
GGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAAA460.33464280517968864No Hit
GGGCGCCTCCTCCGCGGGTATAACCAGTTCGCCTACGACGGCAAGGATTA450.3273679615888258No Hit
GGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTG420.30554343081623747No Hit
GGGAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTC370.26916921286192347No Hit
GGGCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC360.2618943692710607No Hit
GGGCAAGGATTACATCGCCCTGAACGAGGATCTGCGCTCCTGGACCGCCG340.2473446820893351No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG330.24006983849847227No Hit
GGGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTG290.21097046413502107No Hit
GGGATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCA290.21097046413502107No Hit
GGGGTCCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGA290.21097046413502107No Hit
GGGAGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGT290.21097046413502107No Hit
GGGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTT280.2036956205441583No Hit
GGGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCC270.19642077695329552No Hit
GGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC270.19642077695329552No Hit
GGGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAC270.19642077695329552No Hit
GGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACCC270.19642077695329552No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC260.1891459333624327No Hit
GGGCAAGGATTACATCGCCCTGAAAGAGGACCTGCGCTCTTGGACCGCGG260.1891459333624327No Hit
GGGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACC260.1891459333624327No Hit
GGGCCATCAGGTCCGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT250.1818710897715699No Hit
GGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG250.1818710897715699No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTC250.1818710897715699No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT250.1818710897715699No Hit
GGGCCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAG250.1818710897715699No Hit
GGGAGTAACTTACTCCCTATAACTTGGAATGTGGGTGGAGGGGTTCATAG240.1745962461807071No Hit
GGGGCTGCTCTGGCCCCTGGTCCTGTCCTGTTCTCCAGCATGGTGTGTCT230.16732140258984432No Hit
GGGGCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTC230.16732140258984432No Hit
GGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG220.16004655899898151No Hit
GGGGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTGTCCTGTTCTC220.16004655899898151No Hit
GGGGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTGTCCTCTTCTC220.16004655899898151No Hit
GGGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAA220.16004655899898151No Hit
GGGGAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTT220.16004655899898151No Hit
GGGGGAATGTGGGTGGAGGGGTTCATAGTTCTCCCTGAGTGAGACTTGCC210.15277171540811874No Hit
GGGGGGGTGGAGGGGTTCATAGTTCTCCCTGAGTGAGACTTGCCTGCTTC210.15277171540811874No Hit
GGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA210.15277171540811874No Hit
GGGAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTCCA210.15277171540811874No Hit
GGGGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGTC210.15277171540811874No Hit
GGGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCT200.14549687181725593No Hit
GGGCTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCAT200.14549687181725593No Hit
GGGGATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC190.13822202822639315No Hit
GGGCCCTTTCATCAGTCTTCCCGCGTCCGCCGATTCCTCCTCCTTGGTCG190.13822202822639315No Hit
GGGAGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCT190.13822202822639315No Hit
GGGGCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT180.13094718463553034No Hit
GGGGGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTGTCCTGTTCT180.13094718463553034No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG180.13094718463553034No Hit
GGGGGGTGGAGGGGTTCATAGTTCTCCCTGAGTGAGACTTGCCTGCTTCT180.13094718463553034No Hit
GGGAGAATCTCCTCAGACGCCGAGATGCGGGTCACGGCGCCCCGAACCGT170.12367234104466755No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG170.12367234104466755No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG160.11639749745380473No Hit
GGGCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGC160.11639749745380473No Hit
GGGCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGC160.11639749745380473No Hit
GGGAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGTCCTCCTGCTGC160.11639749745380473No Hit
GGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC160.11639749745380473No Hit
GGGGATCAGGTCCGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC160.11639749745380473No Hit
GGGAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCAATT160.11639749745380473No Hit
GGGATTCTCCCCAGAGGCCGAGATGCGGGTCATGGCGCCCCGAGCCCTCC150.10912265386294195No Hit
GGGGATTCTCTTTTCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCT150.10912265386294195No Hit
GGGCTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGC140.10184781027207915No Hit
GGGGCTTCTCTGGCCCCTGGTCCTGTCCTGTTCTCCAGCATGGTGTGTCT140.10184781027207915No Hit
GGGCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCC140.10184781027207915No Hit
GGGGCTCTGGCCCCTGGTCCTGTCCTGTTCTCCAGCATGGTGTGTCTGAG140.10184781027207915No Hit
GGGGGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTGTCCTCTTCT140.10184781027207915No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA140.10184781027207915No Hit
GGGGCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAG140.10184781027207915No Hit
GGGATCAGGTCCGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCA140.10184781027207915No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)