FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13746
Sequences flagged as poor quality0
Sequence length36-200
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCCTCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACG1370.9966535719482031No Hit
GGCTCAACTCGCCGCTGCACTGTGAAGCTCTCACCAACCCCGTAGTTGTG840.6110868616324749No Hit
ACTCCTCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGTACTCCCCCACG800.5819874872690237No Hit
CTCCTCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGT420.30554343081623747No Hit
CCTGTCTTCCAGGAAGTCCTTCTGGCTGTTCCAGTACTCCTCATCAGGCC420.30554343081623747No Hit
GTAATTGTCCACCTGGCCCCGCTTCTGCTCCAGGAGGTCCTTCTGGCTGT390.2837189000436491No Hit
TAGATGCATCTTTCCAGCAACCGCACCCGCTCCGTCCCATTGAAGAAATG340.2473446820893351No Hit
CTGTTCATACCCGCGGAGGAAGCGCCCGTCGGGCCCCAGCTCGCAGCCAT260.1891459333624327No Hit
GAGACAGCGTGGTGAGTCATATGCGTTTTGGGGGCGTCCGTGCGCTGCAG260.1891459333624327No Hit
CTCCTCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGTACTCCCCCACGT240.1745962461807071No Hit
GGTCTGGTGTCCCCAGCCAAAGCCAGTGGGGAGCTCAGCACCATCAGTGT230.16732140258984432No Hit
CCTCCTGGTTATGGAAGTGTCTCTCCAGGAACCGCACCCGCTCCGTCCCA230.16732140258984432No Hit
AGATGCATCTTTCCAGCAACCGCACCCGCTCCGTCCCATTGAAGAAATGA220.16004655899898151No Hit
GGTACAGGAAGTAGGCGGTGGTGGCCTGGCCAGCGAGGAGCAGAGTCACC210.15277171540811874No Hit
ACGCCTCTTCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACG200.14549687181725593No Hit
ACGCCTCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACG190.13822202822639315No Hit
CACGTACTCCTCTTGGTTATAGAAGTATCTGTCCAGGAACCGCACCCGCT190.13822202822639315No Hit
GCACTGTGAAGCTCTCACCAACCCCGTAGTTGTGTCTGCAGTAGGTGTCC170.12367234104466755No Hit
TTCCAGCAACCGCACCCGCTCCGTCCCATTGAAGAAATGACATTCAAACT160.11639749745380473No Hit
TTCCAGCAACCGCACCCGCTCCGTCCCATTGAAGAAATGACACTCAGACG150.10912265386294195No Hit
CCACCGCGGCCCGCCTCTGCTCCAGGAGGTCCTTCTGGCTGTTCCAGTAC150.10912265386294195No Hit
TTATAGAAGTATCTGTCCAGGAACCGCACCCGCTCCGTCCCATTGAAGAA140.10184781027207915No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)