FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15235
Sequences flagged as poor quality0
Sequence length36-90
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCC3202.100426649163111No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTG2811.844437151296357No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTC2671.752543485395471No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA2411.5818838201509682No Hit
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC2131.398096488349196No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC1881.234000656383328No Hit
GGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCAG1791.1749261568756153No Hit
GGGGAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCT1781.1683623235969807No Hit
GGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTGC1601.0502133245815555No Hit
GGGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACT1520.9977026583524778No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT1370.899245159172957No Hit
GGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAACA1350.8861174926156876No Hit
GGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA1210.7942238267148014No Hit
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC1180.7745323268788973No Hit
GGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTG1180.7745323268788973No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT1110.7285854939284542No Hit
GGGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA1080.70889399409255No Hit
GGGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTT960.6301279947489333No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG940.617000328191664No Hit
GGGGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTGT940.617000328191664No Hit
GGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTG930.6104364949130292No Hit
GGGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTG920.6038726616343945No Hit
GGGGGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTG870.5710534952412208No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC820.5382343288480472No Hit
GGGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAC800.5251066622907777No Hit
GGGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCT770.5054151624548736No Hit
GGGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCC760.4988513291762389No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG660.4332129963898917No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG650.426649163111257No Hit
GGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTC630.4135214965539875No Hit
GGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG560.36757466360354446No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA530.3478831637676403No Hit
GGGCCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT500.32819166393173616No Hit
GGGCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGC490.3216278306531014No Hit
GGGATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCA470.308500164095832No Hit
GGGCAAGGATTACATCGCCCTGAACGAGGATCTGCGCTCCTGGACCGCCG450.29537249753856254No Hit
GGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACCC450.29537249753856254No Hit
GGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA430.2822448309812931No Hit
GGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC430.2822448309812931No Hit
GGGCAAGGATTACATCGCCCTGAAAGAGGACCTGCGCTCTTGGACCGCGG410.2691171644240236No Hit
GGGGATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC390.2559894978667542No Hit
GGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC390.2559894978667542No Hit
GGGCGCCTCCTCCGCGGGTATAACCAGTTCGCCTACGACGGCAAGGATTA390.2559894978667542No Hit
GGGGCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTG360.23629799803085No Hit
GGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCAAGCTGTGTTGACT340.2231703314735806No Hit
GGGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACC330.21660649819494585No Hit
GGGAGATCCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTC330.21660649819494585No Hit
GGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG320.21004266491631113No Hit
GGGGCCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTC310.20347883163767644No Hit
GGGGAGATCCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTT300.1969149983590417No Hit
GGGGAGCTCTACTGACTCCCAACAGAGCGCCCAAGAAGAAAATGGCCATA290.19035116508040695No Hit
GGGAGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCT290.19035116508040695No Hit
GGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTT280.18378733180177223No Hit
GGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAA280.18378733180177223No Hit
GGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGC280.18378733180177223No Hit
GGGTCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTC270.1772234985231375No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA260.17065966524450277No Hit
GGGCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC250.16409583196586808No Hit
GGGGTCCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGA250.16409583196586808No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG250.16409583196586808No Hit
GGGAGTTCTAAAGTCCCCACGCACCCACCCGGACTCAAAATCTCCTCAGA240.15753199868723333No Hit
GGGCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCC230.15096816540859861No Hit
GGGACTCCCGAGCTCTACTGACTCCCAACAGAGCGCCCAAGAAGAAAATG230.15096816540859861No Hit
GGGGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTGTCCTGTTCTC230.15096816540859861No Hit
GGGAGACGCCGAGATGCGGGTCACGGCGCCCCGAACCGTCCTCCTGCTGC230.15096816540859861No Hit
GGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAG230.15096816540859861No Hit
GGGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCAAGCTGTGTTGAC230.15096816540859861No Hit
GGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG220.1444043321299639No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTC220.1444043321299639No Hit
GGGGAGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCC220.1444043321299639No Hit
GGGCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTGTGCTCACAGTCA210.13784049885132918No Hit
GGGACAGTTTCAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCAC210.13784049885132918No Hit
GGGGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTC210.13784049885132918No Hit
GGGTCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTT210.13784049885132918No Hit
GGGCCCTTTCATCAGTCTTCCCGCGTCCGCCGATTCCTCCTCCTTGGTCG200.13127666557269443No Hit
GGGACTCGGCCTCAGTTCCTCATCACTGTTCCTGTGCTCACAGTCATCAA200.13127666557269443No Hit
GGGGGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTGTCCTGTTCT200.13127666557269443No Hit
GGGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCT200.13127666557269443No Hit
GGGCCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAG200.13127666557269443No Hit
GGGGACTCAGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGA190.12471283229405973No Hit
GGGAGTAACTTACTCCCTATAACTTGGAATGTGGGTGGAGGGGTTCATAG190.12471283229405973No Hit
GGGGCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT190.12471283229405973No Hit
GGGATTCTCTTTTCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTC190.12471283229405973No Hit
GGGCTGGCTAACATTGCTATATTGAACAACAACTTGAATACCTTGATCCA190.12471283229405973No Hit
GGGCCTGTGCGTGGAGTGGCTCCGCAGATACCTGGAGAACGGGAAGGAGA180.118148999015425No Hit
GGGGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCAG180.118148999015425No Hit
GGGGGTCCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAG170.1115851657367903No Hit
GGGCTTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAACA170.1115851657367903No Hit
GGGGGAGCTCTACTGACTCCCAACAGAGCGCCCAAGAAGAAAATGGCCAT170.1115851657367903No Hit
GGGGAGACGCCGAGATGCGGGTCACGGCGCCCCGAACCGTCCTCCTGCTG170.1115851657367903No Hit
GGGGCTTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAC170.1115851657367903No Hit
GGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT170.1115851657367903No Hit
GGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGTT170.1115851657367903No Hit
GGGAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTCCA170.1115851657367903No Hit
GGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCT160.10502133245815556No Hit
GGGGGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTT160.10502133245815556No Hit
GGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACG160.10502133245815556No Hit
GGGTCAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTAC160.10502133245815556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)