FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11141
Sequences flagged as poor quality0
Sequence length36-90
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC2392.1452293330939773No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA2091.875953684588457No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCC1791.606678036082937No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTC1671.4989677766807288No Hit
GGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTGC1561.4002333722287048No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTG1461.3104748227268648No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC1431.2835472578763127No Hit
GGGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACT1361.2207162732250247No Hit
GGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCAG1221.0950543039224487No Hit
GGGGAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCT1191.0681267390718965No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT1060.9514406247195045No Hit
GGGCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT970.8706579301678485No Hit
GGGGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGT900.8078269455165604No Hit
GGGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTG800.7180683960147204No Hit
GGGAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTC760.6821649762139844No Hit
GGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTG660.5924064267121444No Hit
GGGCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC620.5565030069114083No Hit
GGGATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCA600.5385512970110403No Hit
GGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTC600.5385512970110403No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT590.5295754420608564No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG580.5205995871106723No Hit
GGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTG560.5026478772103044No Hit
GGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA560.5026478772103044No Hit
GGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAACA550.49367202226012025No Hit
GGGGGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTG550.49367202226012025No Hit
GGGGAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTT540.4846961673099363No Hit
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC530.47572031235975226No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC490.4398168925590163No Hit
GGGGCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTC490.4398168925590163No Hit
GGGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA480.43084103760883224No Hit
GGGGGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGTCCTGTTCT460.41288932770846426No Hit
GGGCAAGGATTACATCGCCCTGAACGAGGATCTGCGCTCCTGGACCGCCG450.4039134727582802No Hit
GGGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCT450.4039134727582802No Hit
GGGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAC400.3590341980073602No Hit
GGGCGCCTCCTCCGCGGGTATAACCAGTTCGCCTACGACGGCAAGGATTA380.3410824881069922No Hit
GGGGATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC360.32313077820662417No Hit
GGGGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGTCCTGTTCTC360.32313077820662417No Hit
GGGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCC350.3141549232564402No Hit
GGGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTT320.28722735840588814No Hit
GGGGCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT290.26029979355533617No Hit
GGGCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCC290.26029979355533617No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG280.2513239386051522No Hit
GGGGGAATGTGGGTGGAGGGGTTCATAGTTCTCCCTGAGTGAGACTTGCC270.24234808365496815No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG270.24234808365496815No Hit
GGGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCT250.22439637375460011No Hit
GGGAGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCT240.21542051880441612No Hit
GGGACTTGGGTTCATGGTCTCTAATATTTCAAACAGGAGCTCCCTTTAGC220.1974688089040481No Hit
GGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGACT210.1884929539538641No Hit
GGGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGAC210.1884929539538641No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA210.1884929539538641No Hit
GGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG200.1795170990036801No Hit
GGGACTCGGCCTCAGTTCCTCATCACTGTTCCTGTGCTCACAGTCATCAA200.1795170990036801No Hit
GGGCTGGCTAACATTGCTATATTGAACAACAACTTGAATACCTTGATCCA200.1795170990036801No Hit
GGGAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTCCA190.1705412440534961No Hit
GGGACTCATATTTTTCCCAGACGCGGAGGTTGGGGTCATGGCGCCCCGAA190.1705412440534961No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG190.1705412440534961No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTC190.1705412440534961No Hit
GGGAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCAATT190.1705412440534961No Hit
GGGGAGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCC190.1705412440534961No Hit
GGGGTCCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGA180.16156538910331208No Hit
GGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA180.16156538910331208No Hit
GGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACCC180.16156538910331208No Hit
GGGAGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGT180.16156538910331208No Hit
GGGCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTGTGCTCACAGTCA170.1525895341531281No Hit
GGGCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGC170.1525895341531281No Hit
GGGAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGTCCTCCTGCTGC170.1525895341531281No Hit
GGGACTCCCGAGCTCTACTGACTCCCAACAGAGCGCCCAAGAAGAAAATG170.1525895341531281No Hit
GGGGCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTGTGCTCACAGTC160.14361367920294407No Hit
GGGGAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGTCCTCCTGCTG160.14361367920294407No Hit
GGGGGGTGGAGGGGTTCATAGTTCTCCCTGAGTGAGACTTGCCTGCTTCT160.14361367920294407No Hit
GGGGGAGCTCTACTGACTCCCAACAGAGCGCCCAAGAAGAAAATGGCCAT150.13463782425276008No Hit
GGGGCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAG150.13463782425276008No Hit
GGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC150.13463782425276008No Hit
GGGGAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCAAT150.13463782425276008No Hit
GGGGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGTC150.13463782425276008No Hit
GGGCTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCAT150.13463782425276008No Hit
GGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCT140.1256619693025761No Hit
GGGAGTAACTTCCTCCCTATAACTTGGAATGTGGGTGGAGGGGTTCATAG140.1256619693025761No Hit
GGGGACTCGGCCTCAGTTCCTCATCACTGTTCCTGTGCTCACAGTCATCA140.1256619693025761No Hit
GGGGACTCAGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGA130.11668611435239205No Hit
GGGAGACGCCGAGATGCGGGTCACGGCGCCCCGAACCGTCCTCCTGCTGC130.11668611435239205No Hit
GGGCCCTTTCATCAGTCTTCCCGCGTCCGCCGATTCCTCCTCCTTGGTCG130.11668611435239205No Hit
GGGACATCAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTC130.11668611435239205No Hit
GGGTCAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTAC130.11668611435239205No Hit
GGGCCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAG130.11668611435239205No Hit
GGGCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCC120.10771025940220806No Hit
GGGGGGACTCAGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCC120.10771025940220806No Hit
GGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAG120.10771025940220806No Hit
GGGTCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTC120.10771025940220806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)