FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25987
Sequences flagged as poor quality0
Sequence length36-90
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATAGTTCTCCCTGAGTGAGACTTACCTGCTCCTCTGGCCCCTGGTCC5652.174164005079463No Hit
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC4091.5738638550044253No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTACCTGCTCCTCTGGCCCCTGGTC3911.5045984530726901No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA3681.416092661715473No Hit
GGGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACT2901.1159425866779542No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCC2891.112094508792858No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC2550.9812598606995805No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTC2480.9543233155039058No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT2310.888905991457267No Hit
GGGGGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCTTGTCCTCTTCT2250.8658175241466887No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTG2150.8273367452957249No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCC1920.7388309539385077No Hit
GGGGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCTTGTCCTCTTCTC1850.7118944087428329No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC1730.6657174741216763No Hit
GGGGAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCT1670.6426290068110979No Hit
GGGATAGTTCTCCCTGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCT1660.6387809289260015No Hit
GGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTGC1600.6156924616154231No Hit
GGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCAG1570.6041482279601339No Hit
GGGGGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCT1540.5926039943048448No Hit
GGGGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCTC1520.5849078385346519No Hit
GGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCCTCCTGCTGC1350.5194905144880133No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTC1300.5002501250625313No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT1240.4771616577519529No Hit
GGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTC1160.4463770346711818No Hit
GGGGATAATTTGGAATGTGGGTGGAGGGGGATCATAGTTCTCCCTGAGTG1140.43868087890098895No Hit
GGGATAATTTGGAATGTGGGTGGAGGGGGATCATAGTTCTCCCTGAGTGA1100.42328856736060333No Hit
GGGGGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCT1070.41174433370531416No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG1050.4040481779351214No Hit
GGGGATAGTTCTCCCTGATTGAGACTTGCCTGCTGCTGTGACCACTGGTC1030.39635202216492865No Hit
GGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTG1020.3925039442798322No Hit
GGGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA1010.38865586639473587No Hit
GGGGGGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCC980.37711163273944664No Hit
GGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA980.37711163273944664No Hit
GGGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTG970.37326355485435025No Hit
GGGGGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGTCCTGTTCT830.31939046446300073No Hit
GGGGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGT810.31169430869280795No Hit
GGGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCCTCCTGCTG790.3039981529226151No Hit
GGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACCC780.3001500750375188No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA780.3001500750375188No Hit
GGGGTCATAGTTCTCCCTGAGTGAGACTTACCTGCTCCTCTGGCCCCTGG770.2963019971524224No Hit
GGGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTT730.2809096856120368No Hit
GGGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCT720.2770616077269404No Hit
GGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAAA720.2770616077269404No Hit
GGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTG680.2616692961865548No Hit
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC660.25397314041636204No Hit
GGGAGTTCTCCCTGAGTGAGACTTACCTGCTCCTCTGGCCCCTGGTCCTG650.25012506253126565No Hit
GGGGAGTTCTCCCTGAGTGAGACTTACCTGCTCCTCTGGCCCCTGGTCCT650.25012506253126565No Hit
GGGATCATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGG640.24627698464616926No Hit
GGGGGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTG630.24242890676107282No Hit
GGGGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGTCCTGTTCTC630.24242890676107282No Hit
GGGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCC610.23473275099088006No Hit
GGGGGAGTGAGACTTACCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCT610.23473275099088006No Hit
GGGGGTCATAGTTCTCCCTGAGTGAGACTTACCTGCTCCTCTGGCCCCTG600.23088467310578364No Hit
GGGCCTGTTCTCCAGCATGGTGTGTCTGAAGCTCCCTGGAGGCTCCTGCA590.22703659522068728No Hit
GGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAACA590.22703659522068728No Hit
GGGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCAAGCTGTGTTGAC580.2231885173355909No Hit
GGGGATCATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTG570.21934043945049447No Hit
GGGATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTCGTCTCA560.21549236156539808No Hit
GGGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACC560.21549236156539808No Hit
GGGCCCCATAATTTGGAATGTGGGTGGAGGGGGATCATAGTTCTCCCTGA530.2039481279101089No Hit
GGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACT520.2001000500250125No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTG520.2001000500250125No Hit
GGGAGATCCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTC510.1962519721399161No Hit
GGGAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTC500.1924038942548197No Hit
GGGGAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCT490.18855581636972332No Hit
GGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCAAGCTGTGTTGACT490.18855581636972332No Hit
GGGCTCACAATTGCTCTACAGCTCAGAACAGCAACTGCTGAGGCTGCCTT490.18855581636972332No Hit
GGGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAA460.17701158271443412No Hit
GGGATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCA450.17316350482933773No Hit
GGGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAC450.17316350482933773No Hit
GGGAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTCCA450.17316350482933773No Hit
GGGGCTCACAATTGCTCTACAGCTCAGAACAGCAACTGCTGAGGCTGCCT440.16931542694424137No Hit
GGGGAGTGAGACTTACCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCTC440.16931542694424137No Hit
GGGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCT440.16931542694424137No Hit
GGGAGTTCTCCCTGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCTTG440.16931542694424137No Hit
GGGCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTCGTCT440.16931542694424137No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC430.16546734905914495No Hit
GGGATATAGTTCTCCCTGAGTGAGACTTACCTGCTCCTCTGGCCCCTGGT430.16546734905914495No Hit
GGGGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTGT400.15392311540385578No Hit
GGGGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTC400.15392311540385578No Hit
GGGCCCTTTCATCAGTCTTCCCGCGTCCGCCGATTCCTCCTCCTTGGTCG390.1500750375187594No Hit
GGGCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGC380.14622695963366297No Hit
GGGGCCCCATAATTTGGAATGTGGGTGGAGGGGGATCATAGTTCTCCCTG380.14622695963366297No Hit
GGGGGGAGAGGGGTCATAGTTCTCCCTGAGTGAGACTTACCTGCTCCTCT370.1423788817485666No Hit
GGGGGAAATGTGGGTGGAGAGGGGTCATAGTTCTCCCTGAGTGAGACTTA370.1423788817485666No Hit
GGGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGAC360.1385308038634702No Hit
GGGGGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTG350.1346827259783738No Hit
GGGGCCTGTTCTCCAGCATGGTGTGTCTGAAGCTCCCTGGAGGCTCCTGC350.1346827259783738No Hit
GGGGATATAGTTCTCCCTGAGTGAGACTTACCTGCTCCTCTGGCCCCTGG350.1346827259783738No Hit
GGGGGAGAGGGGTCATAGTTCTCCCTGAGTGAGACTTACCTGCTCCTCTG350.1346827259783738No Hit
GGGGAGACTTACCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCTCCAGC340.1308346480932774No Hit
GGGATCCACAGGTTTTTATTCTTTCTGCCAGGTACATCAGATCCATCAGG340.1308346480932774No Hit
GGGGAAATGTGGGTGGAGAGGGGTCATAGTTCTCCCTGAGTGAGACTTAC340.1308346480932774No Hit
GGGCCCTATAATTTGAAATGTGGGTGGAGAGGGGTCATAGTTCTCCCTGA330.12698657020818102No Hit
GGGGAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTT330.12698657020818102No Hit
GGGCCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT330.12698657020818102No Hit
GGGGGGTCCTGTCCTGTTCTCCAGCATGGTGTGTCTGAAGCTCCCTGGAG320.12313849232308463No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG320.12313849232308463No Hit
GGGGATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTCGTCTC310.11929041443798823No Hit
GGGGAGTTCTAAAGTCCCCACGCACCCACCCGGACTCAGAATCTCCTCAG300.11544233655289182No Hit
GGGGGTCCTGTCCTGTTCTCCAGCATGGTGTGTCTGAAGCTCCCTGGAGG300.11544233655289182No Hit
GGGCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTCGTCTC300.11544233655289182No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA290.11159425866779545No Hit
GGGAGTTCTAAAGTCCCCACGCACCCACCCGGACTCAGAATCTCCTCAGA290.11159425866779545No Hit
GGGCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCC280.10774618078269904No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG280.10774618078269904No Hit
GGGGCCCTTTCATCAGTCTTCCCGCGTCCGCCGATTCCTCCTCCTTGGTC280.10774618078269904No Hit
GGGGACTCGGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGA280.10774618078269904No Hit
GGGCTCACAATTACTCTACAGCTCAGAACACCAACTGCTGAGGCTGCCTT280.10774618078269904No Hit
GGGGGACTCGGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCG270.10389810289760265No Hit
GGGCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGC270.10389810289760265No Hit
GGGCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTGTGCTCACAGTCA260.10005002501250625No Hit
GGGCCCATAATTTGGAATGTGGGTGGAGGGGGATCATAGTTCTCCCTGAG260.10005002501250625No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)