FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25987
Sequences flagged as poor quality0
Sequence length36-200
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGT1190.45792126832647095No Hit
GTGCAGATACCGCACCCGCTCCGTCCCGTTGAAGAAATGACACTCAAACT770.2963019971524224No Hit
CTGCACACGGTGTCCACCTCGGCCCGCCTCCGCTCCAGGAAGTCCTTCTG720.2770616077269404No Hit
GTGCAGATACCGCACCCGCTCCGTCCCGTTGAAGAAATGACACTCATACT580.2231885173355909No Hit
GCACACGGTGTCCACCTCGGCCCGCCTCCGCTCCAGGAAGTCCTTCTGGC570.21934043945049447No Hit
GCCACCGGGGTGCTCAGCATCGCCAGCATCAAGGTCACAGTTGCTACCCG500.1924038942548197No Hit
CCTGTCTTCCAGGAAGTCCTTCTGGCTGTTCCAGTACTCAGCGTCAGGCC450.17316350482933773No Hit
GGCTCAACTCGCCGCTGCACTGTGAAGCTCTCACCAACCCCGTAGTTGTG420.16161927117404856No Hit
CTCCCTCTTAGGCTGCCACAGGAAACGTGGTCGGGTGTCCCCAGACAAAG410.15777119328895217No Hit
GCCACCGGGGTGCTCAGCATCGCCAGCATCAAGGTGACAGTTGCTACCCG400.15392311540385578No Hit
CTCCACAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCCCGCG360.1385308038634702No Hit
CCAGTACTCAGCGTCAGGCCGCCCCAGCTCCGTCACCGCCCGGTACTCCC340.1308346480932774No Hit
GATACACAGTCACCTTAGGTTGGACTCGCCGCTGCACTGTGAAGCTCTCC330.12698657020818102No Hit
GATACACAGTCACCTCAGGATGGACTCGCCTCTGCACTGTGAAGCTCTCA310.11929041443798823No Hit
CCTCCTGGTTATAGAAGTATCTGTCCAGGAACCGCACCCGCTCCGTCCCA310.11929041443798823No Hit
CTCACCAACCCCGTAGTTGTGTCTGCACACGGTGTCCACCTCGGCCCGCC300.11544233655289182No Hit
CTGTTCATACCCGCGGAGGAAGCGCCTGTCGGGCCCCAGCTCGCAGCCAT270.10389810289760265No Hit
CTGCACACGGTGTCCACCGCGGCGCGCCTGTCTTCCAGGAAGTCCTTCTG270.10389810289760265No Hit
GGTACAGGAAGTAGGCGGTGGTGGCCTGGCCAGCGAGGAGCAGAGTCACC270.10389810289760265No Hit
GATGCCTCTGTGCAGATACCGCACCCGCTCCGTCCCGTTGAAGAAATGAC270.10389810289760265No Hit
CGCCCGATACACCCCCACGTCGCTGTCGAAGCGTGCGTACTCCTCTCGGT270.10389810289760265No Hit
GCCTCCGCTCCAGGAGGTCCTTCTGGCTGTTCCAGTACTCAGCGTCAGGC270.10389810289760265No Hit
CTCCACAACCCCGTAGTTGTATCTGCAGTAGGTGTCCACCTCGGCCCGCC260.10005002501250625No Hit
CTGCACACGGTGTCCACCGCGGCCCGCGCCTGCTCCAGGATGTCCTTCTG260.10005002501250625No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)