FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004963880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374626
Sequences flagged as poor quality0
Sequence length36-90
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT89882.3991927949474943No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC77932.080207994106122No Hit
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC45551.2158793036254825No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA42831.1432735581620068No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG38111.017281235151858No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG23960.6395711990091452No Hit
GGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT23380.6240890915206099No Hit
GGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA22700.6059376551547411No Hit
GGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG22570.6024675276142072No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG21040.5616267957910023No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT19120.5103756813461959No Hit
GGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAG17990.4802122650323256No Hit
GGGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAAC17650.47113654684939116No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA17150.45778990246272283No Hit
GGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAA16770.4476464527288549No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG16420.4383038016581871No Hit
GGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC15310.4086742511197835No Hit
GGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG14440.3854510898869806No Hit
GGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCA13580.36249486154191113No Hit
GGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGC12240.3267258545856401No Hit
GGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTT11410.3045704249037707No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC11000.2936261765067027No Hit
GGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGA10450.27894486768136756No Hit
GGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG10080.269068350835233No Hit
GGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC9970.266132089070166No Hit
GGGCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTCTGG9330.24904838425523057No Hit
GGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACG8300.22155429681869387No Hit
GGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCTC8050.21488097462535968No Hit
GGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGTT7860.20980924975842574No Hit
GGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGGCCTC7550.20153433023869138No Hit
GGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAG7240.19325941071895703No Hit
GGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTGGTGC6950.18551835697468944No Hit
GGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATG6850.18284902809735576No Hit
GGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGT6740.17991276633228873No Hit
GGGGCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTCTG6530.17430717568988804No Hit
GGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCA6510.17377330991442133No Hit
GGGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAA6490.17323944413895456No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6400.17083704814935427No Hit
GGGCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGCG6310.16843465215975398No Hit
GGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT5880.15695653798721926No Hit
GGGAGCCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTC5850.15615573932401916No Hit
GGGTGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT5790.15455414199761897No Hit
GGGCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAG5750.1534864104466855No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT5620.15001628290615174No Hit
GGGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTGGTG5590.14921548424295164No Hit
GGGCCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGC5510.1470800211410847No Hit
GGGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCT5450.1454784238146845No Hit
GGGCCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCTA5410.14441069226375106No Hit
GGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGTGCATA5290.14120749761095064No Hit
GGGCTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCAT4780.12759392033654898No Hit
GGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACAGTGC4760.12706005456108227No Hit
GGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGGCCT4700.12545845723468205No Hit
GGGCTTTTTCGTCTGGGCTGCCAACATGCCATCCAGACTGAGGAAGACCC4620.12332299413281513No Hit
GGGACAGCAGTCCGTGCCGCCGTCCCGCCCGCCAGCGCCCCAGCGAGGAA4620.12332299413281513No Hit
GGGCCCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCT4530.12092059814321483No Hit
GGGATGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT4440.11851820215361454No Hit
GGGCTTTTCACAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCCT4350.11611580616401425No Hit
GGGGCCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCT4310.11504807461308077No Hit
GGGGCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGC4140.11051021552161355No Hit
GGGCTCTTTCCCATCTTGCAAGATGGCGGGTGAAAAAGTTGAGAAGCCAG4110.10970941685841346No Hit
GGGCCTTTCGTTGCCTGATCGCCGCCATCATGGGTCGCATGCATGCTCCC4030.10757395375654652No Hit
GGGGCTCTTTCCCATCTTGCAAGATGGCGGGTGAAAAAGTTGAGAAGCCA3950.1054384906546796No Hit
GGGGCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGA3940.10517155776694623No Hit
GGGCTTTTCCTGTGGCAGCAGCCGGGCTGAGAGGAGCGTGGCTGTCTCCT3910.10437075910374614No Hit
GGGCTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGC3890.10383689332827939No Hit
GGGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGTGCAT3890.10383689332827939No Hit
GGGGAGCCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTT3820.10196836311414585No Hit
GGGGCCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAG3820.10196836311414585No Hit
GGGCTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAGAAGGGTGGC3770.10063369867547901No Hit
GGGCTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTT3760.10036676578774564No Hit
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC3750.10009983290001229No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)