FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16180
Sequences flagged as poor quality0
Sequence length36-200
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTTTGCCATGTAGGAACCTCCAGGGAGCTTCAGACACACCATGCTGG420.2595797280593325No Hit
GATACACAGTCACCTTAGGCTCAACTCGCCGCTGCACTGTGAAGCTCTCC350.21631644004944375No Hit
GGCTCAACTCGCCGCTGCACTGTGAAGCTCTCACCAACCCCGTAGTTGTG340.21013597033374534No Hit
GGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCA330.20395550061804696No Hit
CCACCGCGGCCCGCGCCTGCTCCAGGATGTCCTTCTGGCTGTTCCAGTAC330.20395550061804696No Hit
CACGTTCTCCTCTTGGTTATAGATGCCTCTGTGCAGGAACCGCACCCGCT330.20395550061804696No Hit
GCATCAGGCCGCCCCTGCGGCGTCACCGCGCGGTACACCCCCACGTCGCT310.19159456118665016No Hit
CACCCCGCACGCGCTCCGTCCCGTTGGTGAAGTAGCACAGGCCCTTAAAC300.1854140914709518No Hit
GATACACAGTCACCTTAGGCTCAACTCGCCGCTGCACTGTGAAGCTCTCA290.1792336217552534No Hit
CTCCACAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCCCGCG290.1792336217552534No Hit
CTCCACAGCCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCGCGCC290.1792336217552534No Hit
GCTCCAGGATGTCCTTCTGGCTGTTCCAGTACTCAGCGTCAGGCCGCCCC280.173053152039555No Hit
GGCTCAACTCGCCGCTGCACTGTGAAGCTCTCCACAACCCCGTAGTTGTG270.16687268232385663No Hit
CCTCCTGGTTATAGAAGTATCTGTCCAGGAACCGCACCCGCTCCGTCCCA270.16687268232385663No Hit
CACCCCGCACGCGCTCCGTCCCGTTGGTGAAGTAGCACATGCCCTTAAAC260.16069221260815822No Hit
GTCACGGCCTCGTCCAGCTCGTAGTTGTGTCTGCATACCCTGTCCGGCAC260.16069221260815822No Hit
GGTTGGACTCGCCGCTGCACTGTGAAGCTCTCACCAACCCCGTAGTTGTG250.15451174289245984No Hit
CAAGTCCTCCTCTTGGTTATAGATGTCTCTGTGCAGGAACCGCACCCGCT250.15451174289245984No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC240.14833127317676142No Hit
GAGACAGCGTGGTGAGTCATATGCGTCTTGGGGGCGTCCGTGCGCTGCAG230.14215080346106304No Hit
CTCACCAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCGCGCC230.14215080346106304No Hit
GATGCCTCTGTGCAGGAACCGCACCCGCTCCGTCCCGTTGAAGAAATGAC220.13597033374536466No Hit
GCACATCCTGTCCGGCACTGCCCGCTTCTCCTCCAGGATGTCCTTCTGGC220.13597033374536466No Hit
GGGCCCGTTGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA220.13597033374536466No Hit
GCATACCCTGTCCGGCACTGCCCGCTTCTCCTCCAGGAGGTCCTTCTGGC220.13597033374536466No Hit
GGCTCAACTCGCCGCTGCACTGTGAAGCTCTCCACAGCCCCGTAGTTGTG210.12978986402966625No Hit
CTGTCCAGGAACCGCACCCGCTCCGTCCCATTGAAGAAATGACACTCCCT210.12978986402966625No Hit
GGACTTCCTTCTGGCTGTTCCAGTACTCGGCGCTAGGCCGCCCCTGCGGC200.12360939431396785No Hit
GTCACGGCCTCGTCCAGCTCGTAGTTGTGTCTGCACATCCTGTCCGGCAC200.12360939431396785No Hit
GCACATCCTGTCCGGCACTGCCCGCTTCTCCTCCAGGAGGTCCTTCTGGC190.11742892459826948No Hit
GCGCTAGGCCGCCCCTGCGGCGTCACCGCCCGGTACACCCCCACGTCGCT180.11124845488257107No Hit
CTCCACAGCCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCCCGCG180.11124845488257107No Hit
ACTCTGACCCCTCCTGCTCCATCCACGGCGCCCGCGGCACCATCCTCGGA180.11124845488257107No Hit
GCATCAGGCCGCCCCTGCGGCGTCACCGCCCGGTACACCCCCACGTCGCT170.10506798516687267No Hit
CTCCCTCTTAGGCTGCCACAGGAAACGTGGTCGGGTGTCCCCAGCCAAAG170.10506798516687267No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)