FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451476387
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC81065491.7955643381189723Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG11693000.0110.959699
GAGCGTC12104750.0107.029028
AGAGCGT12521300.0103.569197
ATCGGAA12827600.0103.43581
TCGGAAG12895200.0102.7393652
CGGAAGA13095500.0100.8946843
AAGAGCG13061750.099.6331256
GAAGAGC15887600.082.472375
GGAAGAG19420050.068.229874
CGCCGTA9885250.026.78729655-59
TCGCCGT10063850.026.2395155-59
GCCGTAT10103350.026.15025955-59
GTCGCCG10178100.025.78742255-59
CCGTATC10251900.025.49948755-59
TGGTCGC10458800.025.3620650-54
CGTATCA10329800.025.32353460-64
GGTCGCC10387450.025.24787750-54
GTGGTCG10671050.024.84511650-54
TCTCGGT10978650.024.35787445-49
CTCGGTG11130750.024.00733245-49
Produced by FastQC (version 0.11.5)