FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440538765
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG33855270.7684969562213214TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA5759450.093.20281
TCGGAAG5894450.091.148372
CGGAAGA6120650.087.809023
AGAGCAC8434150.063.715187
AGCACAC8663750.062.0340969
GAGCACA8795200.061.1111568
GAAGAGC9058100.059.5135425
AAGAGCA11623200.046.5878876
GGAAGAG12657850.042.9786644
TATGCCG4287100.025.44268645-49
CTCGTAT4301050.024.52551340-44
CGTATGC4431500.024.42619745-49
TCTCGTA4339200.024.29551940-44
TCGTATC4496700.024.18327535-39
ATGCCGT4535950.024.05580545-49
TGCCGTC4496500.023.87095845-49
GCCGTCT4524400.023.8512150-54
TATCTCG4409100.023.69959640-44
TCGTATG4420750.023.47383740-44
ATCTCGT4546850.023.19138740-44
Produced by FastQC (version 0.11.5)