FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428875347
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG13013660.30343688652264733TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3295050.065.199061
TCGGAAG3387250.063.464092
CGGAAGA3561550.060.3586463
AGAGCAC5889350.036.729457
AGCACAC6117750.035.3245249
GAGCACA6246000.034.689048
GAAGAGC6455450.033.6791885
AAGAGCA8915450.024.6447686
GGAAGAG9872700.022.3377514
TATGCCG1975250.022.0355845-49
CTCGTAT2003200.020.5224340-44
CGTATGC2107650.020.40371345-49
TCTCGTA2048450.020.05918740-44
TGCCGTC2185050.019.62589545-49
ATGCCGT2230300.019.53971145-49
GCCGTCT2221300.019.297850-54
TATCTCG2123150.019.08714940-44
TCGTATG2157450.018.80586640-44
ATCTCGT2268400.018.1589340-44
CGTCTTC2477350.017.65436750-54
Produced by FastQC (version 0.11.5)