FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428875347
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC13766270.3209853421581726Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3037850.070.4324959
GAGCGTC3378800.063.400318
AGAGCGT3737800.057.4925777
ATCGGAA3816000.056.885951
TCGGAAG3911750.055.4339562
CGGAAGA4130000.052.499243
AAGAGCG4176150.051.7319376
GAAGAGC6948850.031.5283455
CGCCGTA1897700.022.62458255-59
GGAAGAG10246450.021.7251344
TCGCCGT2006800.021.37364255-59
GTCGCCG2121500.020.24745455-59
GCCGTAT2132850.020.21788655-59
TGGTCGC2249550.019.32382450-54
CCGTATC2251550.018.92275655-59
GGTCGCC2281350.018.82122650-54
GTGGTCG2360350.018.47568950-54
CGTATCA2337350.018.3651860-64
GTACGTC2423700.017.7584230-34
TACGTCC2511100.017.3256930-34
Produced by FastQC (version 0.11.5)