FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347934774
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1154150.058.7903371
TCGGAAG1503600.057.09772
CGGAAGA1681450.051.1814653
AGAGCAC3647350.024.1604277
AGCACAC3854050.022.8815299
CGCGCAT831950.022.36425835-39
GAGCACA3966050.022.2809628
TCGCGCA854600.022.0035535-39
GAAGAGC4050100.021.8347765
TCTCGCG895150.021.24608630-34
TATGCCG948100.019.97259545-49
GCGCATC974850.019.27896735-39
CGTATGC1059200.017.8011545-49
CTCGTAT955500.017.26969340-44
TCTCGTA979050.016.62660840-44
ACTCTCG1168950.016.46778130-34
CTCGCGC1048500.016.40214230-34
ATGCCGT1174200.016.06634945-49
GCCGTCT1207300.015.34125550-54
CGCATCT1076650.015.190935-39
Produced by FastQC (version 0.11.5)