FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382157431
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG7055610.18462574393849743TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1649500.076.935531
TCGGAAG1790350.070.824622
CGGAAGA1986300.063.724483
AGAGCAC4144950.030.9105227
AGCACAC4424600.028.9355759
GAGCACA4557600.028.1627468
GAAGAGC4615650.027.8874555
TATGCCG1231700.021.05522745-49
GCGCATC1243400.020.47791135-39
TAATGCG1308850.019.87615230-34
AAGAGCA6708100.019.4792396
CGTATGC1330650.019.2378245-49
CTCGTAT1219400.019.13195240-44
AATGCGC1348500.018.83263830-34
TGCGCAT1375500.018.52390735-39
TCTCGTA1256800.018.32728640-44
ATGCGCA1414600.018.10004235-39
ATGCCGT1458750.017.83867545-49
TGCCGTC1477700.017.1360145-49
CGCATCT1349750.017.11889835-39
Produced by FastQC (version 0.11.5)