FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446743774
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT8176710.18302907563296003TruSeq Adapter, Index 19 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG6842530.15316452960797167TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1643300.080.3369141
ATCGGAA2680600.049.573892
TCGGAAG2834200.046.8974883
CGGAAGA3052700.043.6535644
AGAGCAC5614700.024.0709788
TATGCCG2175050.023.35945145-49
GAGCACA6026950.022.4124779
GAAGAGC6135600.022.1679066
CTCGTAT2170850.021.97360640-44
CGTATGC2301250.021.92160245-49
TCTCGTA2217300.021.5616840-44
TATCTCG2262600.021.00181440-44
AGCACAC5904800.020.9217199
ATGCCGT2443950.020.7667545-49
GCCGTCT2499200.020.11888550-54
ATCTCGT2438200.019.68015940-44
ACACGTC2708550.018.85661310-14
CGTCTGA2701200.018.77801515-19
CGTCTTC2799650.018.17771750-54
CCGTCTT2833150.017.9433450-54
Produced by FastQC (version 0.11.5)