FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489123844
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG13696410.2800192664498278TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2254550.099.419541
TCGGAAG2420850.092.5247342
CGGAAGA2712450.082.420093
AGAGCAC5583650.040.3780027
AGCACAC5975250.037.7462849
GAGCACA6092700.037.0258068
GAAGAGC6109250.037.023125
AAGAGCA8903750.025.6422986
TATGCCG1954950.023.31742545-49
GGAAGAG9928150.023.1624684
CTCGTAT1986200.021.63577540-44
CGTATGC2104050.021.41216345-49
TCTCGTA2027600.021.1854340-44
ATGCCGT2278450.020.12767445-49
TGCCGTC2249000.020.1101445-49
TCGTATG2156300.019.76766440-44
GCCGTCT2294350.019.6469950-54
CCGGAGA2346750.019.32240530-34
ACTCCGG2386150.019.15464630-34
CGTCTGA2390300.019.04663315-19
Produced by FastQC (version 0.11.5)