FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457620937
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC17248140.37690889129926325Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3261050.082.258889
GAGCGTC3647300.073.593348
ATCGGAA3940550.070.003961
TCGGAAG4104050.066.911172
AGAGCGT4055300.066.4214257
CGGAAGA4360250.062.761743
AAGAGCG4619000.058.8962366
GAAGAGC7454200.037.049455
GGAAGAG11198950.025.0039844
CGCCGTA2542450.021.39177955-59
TCGCCGT2692250.020.21012955-59
GCCGTAT2787550.019.60601455-59
GTCGCCG2875550.018.96871855-59
CCGTATC2900750.018.49167355-59
TGGTCGC2999550.018.29976750-54
CGTATCA2980350.018.26627260-64
GGTCGCC3050850.017.685750-54
GTGGTCG3160600.017.46678550-54
GCGTCGT3135150.017.38043410-14
CGTCGTG3190300.017.11813410-14
Produced by FastQC (version 0.11.5)