FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435142935
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG142162213.2670232828208507TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA20411100.0113.452291
TCGGAAG20739400.0111.890472
CGGAAGA20933900.0110.806523
AGAGCAC22808000.0101.75277
AGCACAC22905800.0100.981779
GAGCACA23054000.0100.569368
GAAGAGC23605650.098.319285
AAGAGCA25921000.089.50116
GGAAGAG26979550.086.226644
TATGCCG17535100.026.57219145-49
CTCGTAT17459750.026.20994840-44
ATGCCGT17814700.026.14521845-49
TCTCGTA17567850.026.10577440-44
CGTATGC17676000.025.9907345-49
TGCCGTC17832700.025.85705245-49
TCGTATG17566050.025.84642240-44
CGTCTTC18036700.025.79473150-54
GCCGTCT17841950.025.76532650-54
CCGTCTT18089500.025.68962950-54
ATCTCGT17837650.025.68409540-44
Produced by FastQC (version 0.11.5)