FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435142935
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC146620023.3694680117005693Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG19804850.0116.8403249
GAGCGTC20162650.0114.7493748
AGAGCGT20612750.0112.277677
ATCGGAA20733650.0111.766141
TCGGAAG20957900.0110.4718252
AAGAGCG21186800.0109.369196
CGGAAGA21191600.0109.2270663
GAAGAGC23820350.097.1505055
GGAAGAG27193050.085.2911764
CGCCGTA17085800.027.06158655-59
GCCGTAT17252700.026.79416555-59
TCGCCGT17268500.026.75512355-59
CCGTATC17324650.026.41023655-59
TGGTCGC17579350.026.35836450-54
CGTATCA17383850.026.3349560-64
GTCGCCG17463550.026.33060555-59
GGTCGCC17554600.026.2082150-54
GTGGTCG17857200.025.98526450-54
TCTCGGT18108550.025.67585645-49
CTCGGTG18293200.025.46256845-49
Produced by FastQC (version 0.11.5)