FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477454970
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG8020180.1679777257319156TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1944400.067.459861
TCGGAAG1964000.066.61252
CGGAAGA2313200.056.5569153
AGAGCAC4810100.027.5694647
AGCACAC5129150.025.8545519
GAGCACA5221750.025.4612858
GAAGAGC5585900.023.96365
TATGCCG1463200.018.36926345-49
AAGAGCA8256550.016.4511016
CGTATGC1623950.016.44204345-49
CTCGTAT1577800.016.05692140-44
TCTCGTA1601050.015.78300640-44
TTACTCG1693600.015.7480730-34
TACTCGA1719000.015.58634335-39
GGAAGAG8867050.015.4466754
ATGCCGT1804300.015.08136545-49
TGCCGTC1792550.014.94651845-49
GCCGTCT1793550.014.88803450-54
TCGTATG1744850.014.37758540-44
ACACGTC1919400.014.11319810-14
Produced by FastQC (version 0.11.5)