FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391709545
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG94601512.41509330593412TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA12770350.0117.3607561
TCGGAAG12921350.0116.139762
CGGAAGA13155050.0113.927093
AGAGCAC15004500.099.44957
AGCACAC15150150.098.476669
GAGCACA15316550.097.468818
GAAGAGC15630250.095.785345
AAGAGCA17646100.084.75356
GGAAGAG18607900.080.744124
TATGCCG11107850.027.04956245-49
CTCGTAT10985700.026.80750840-44
TCTCGTA11058250.026.64828140-44
CGTATGC11245150.026.37018845-49
ATGCCGT11421200.026.32634745-49
TCGTATG11138050.026.2232140-44
ATCTCGT11317850.026.02103240-44
TGCCGTC11514100.025.90115745-49
GCCGTCT11459100.025.84254550-54
CGTCTTC11655800.025.77092650-54
CCGTCTT11644650.025.74031650-54
Produced by FastQC (version 0.11.5)