FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488522389
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG64324161.3167085367708706TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA9596800.0106.257631
TCGGAAG9730850.0104.7211152
CGGAAGA10008300.0101.7283863
AGAGCAC12381950.081.9835057
AGCACAC12624500.080.3662349
GAGCACA12831000.079.128848
GAAGAGC13175050.077.321015
AAGAGCA15784400.064.554746
GGAAGAG17023350.060.1548084
TATGCCG7790400.026.22740245-49
CTCGTAT7611500.025.90592840-44
TCTCGTA7656750.025.72972940-44
CGTATGC7912350.025.51769845-49
ATGCCGT8106150.025.2778845-49
TCGTATG7807700.025.0638640-44
TGCCGTC8135900.024.96660445-49
GCCGTCT8113950.024.86466850-54
ATCTCGT7940100.024.79784440-44
CGTCTTC8368500.024.46230750-54
CCGTCTT8432700.024.23652350-54
Produced by FastQC (version 0.11.5)