FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488522389
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC63079571.291231915268473Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG9051550.0109.1761869
GAGCGTC9478100.0104.387858
AGAGCGT9876250.0100.34187
ATCGGAA10150550.099.85491
TCGGAAG10292150.098.250342
CGGAAGA10575250.095.598763
AAGAGCG10611850.094.8229456
GAAGAGC13697200.073.8205645
GGAAGAG17602550.057.81914
CGCCGTA7450150.026.33374255-59
TCGCCGT7530450.025.67112755-59
GCCGTAT7697150.025.5510555-59
TGGTCGC7838400.025.09517550-54
GTCGCCG7801400.025.03323655-59
CCGTATC7811200.024.98419455-59
CGTATCA7815450.024.81729560-64
GGTCGCC7957250.024.71397450-54
GTGGTCG8023950.024.53817650-54
TCTCGGT8550550.023.5846745-49
TCGGTGG8637050.023.20005845-49
Produced by FastQC (version 0.11.5)