FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456322108
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG5508430.12071363415072583TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1970900.044.840851
TCGGAAG2075500.042.6522062
CGGAAGA2346450.037.8294873
AGAGCAC4806700.018.933647
AGCACAC5133350.017.8488569
GAGCACA5279650.017.3515158
GAAGAGC5491250.016.716085
TATGCCG1226700.015.01102245-49
CTCGTAT1274050.014.17436840-44
CGTATGC1326700.013.73968745-49
TCTCGTA1336900.013.468966540-44
TTACTCG1381450.013.36123330-34
TCGTATG1423650.012.79386340-44
TACTCGA1440750.012.71357735-39
AAGAGCA7932450.011.8475486
ATGCCGT1614600.011.6112245-49
TGCCGTC1664450.011.32008445-49
ATCTCGT1624150.011.2269740-44
GCCGTCT1725800.010.80491150-54
GGAAGAG8895150.010.7268924
Produced by FastQC (version 0.11.5)