FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475169414
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC60698821.2774142908112347Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG8104800.0117.5073249
ATCGGAA8535900.0112.714551
GAGCGTC8494350.0112.705218
TCGGAAG8699750.0110.708662
AGAGCGT8889000.0107.97847
CGGAAGA9091900.0105.9071963
AAGAGCG9369250.0102.4838266
GAAGAGC12344000.078.1481555
GGAAGAG15710300.061.6702164
CGCCGTA7557750.025.42585255-59
GCCGTAT7795100.024.68549755-59
TCGCCGT7882150.024.3525855-59
CCGTATC7929050.024.16358855-59
CGTATCA7928050.024.0355960-64
TGGTCGC8122000.023.85561250-54
GCGTCGT8167050.023.5371110-14
GTGGTCG8262000.023.46593350-54
GTCGCCG8137500.023.46431555-59
GGTCGCC8184600.023.42248750-54
CGTCGTG8249100.023.3565710-14
Produced by FastQC (version 0.11.5)