FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412805313
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC54552321.3215023712642961Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG8327800.0103.752389
GAGCGTC8705900.099.233098
AGAGCGT9091950.095.359227
ATCGGAA9381250.093.49621
TCGGAAG9498050.092.3697742
CGGAAGA9634550.090.922913
AAGAGCG9669650.090.367746
GAAGAGC12295800.071.3451465
GGAAGAG15696900.056.17374
TCCGATC518450.027.1191881
CGCCGTA6510000.026.330155-59
TCGCCGT6565450.025.7504255-59
GCCGTAT6692850.025.63436755-59
CCGTATC6791250.024.98434455-59
GTCGCCG6846800.024.96311455-59
CGTATCA6836150.024.88174460-64
TGGTCGC6924450.024.82565550-54
GGTCGCC7012900.024.38725950-54
GTGGTCG7102650.024.27383450-54
TCTCGGT7453900.023.40755345-49
Produced by FastQC (version 0.11.5)