FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479749577
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1399600.030.6169931
TCGGAAG1565550.027.501312
CGGAAGA1793250.023.9850463
TATGCCG789200.011.443727545-49
AGAGCAC4536200.010.0251137
CGTATGC937400.09.6654545-49
CTCGTAT845300.09.66310840-44
AGCACAC4858800.09.5114599
TCTCGTA892400.09.24734340-44
GAGCACA5019450.09.2156958
GAAGAGC5158000.09.0160625
ATGCCGT1096450.08.51732245-49
TGCCGTC1131950.08.00295345-49
GCCGTCT1170250.07.877083350-54
TCGTATG1033400.07.873354440-44
ATCTCGT1141550.07.391649740-44
CGTCTGA1411750.06.676203315-19
ACACGTC1432100.06.60273410-14
CGTCTTC1470100.06.535765650-54
CCGTCTT1505700.06.43131650-54
Produced by FastQC (version 0.11.5)