FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479749577
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1900600.022.6294239
GAGCGTC2313150.018.7077378
ATCGGAA2502300.017.5734331
TCGGAAG2694000.016.3493122
AGAGCGT2666700.016.3415187
CGGAAGA2905800.015.1465723
AAGAGCG3144450.013.9969926
CGCCGTA1100250.08.18242855-59
GAAGAGC6292900.07.5595525
TCGCCGT1255200.07.265888755-59
GCCGTAT1338050.06.930842455-59
GTCGCCG1459500.06.32827755-59
CCGTATC1469600.06.19501755-59
CGTATCA1533300.06.15898360-64
TGGTCGC1563550.05.99044750-54
GGTCGCC1650700.05.563521450-54
GTGGTCG1710650.05.549899650-54
CAGTACG1783950.05.050420830-34
GGAAGAG10188250.05.01656964
Produced by FastQC (version 0.11.5)