FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445537281
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1310950.040.115321
TCGGAAG1457550.036.158182
CGGAAGA1673850.031.5800763
TATGCCG769700.014.14607445-49
AGAGCAC4219150.012.9943157
AGCACAC4505800.012.2239589
CGTATGC902500.012.029181545-49
GAGCACA4633450.011.9482088
CTCGTAT828000.011.65117340-44
GAAGAGC4763650.011.6170695
TCTCGTA868400.011.1174840-44
ATGCCGT1041450.010.63724645-49
TATCTCG932700.010.2406840-44
TGCCGTC1059450.010.2238845-49
GCCGTCT1105700.09.863029550-54
TCGTATG997450.09.52357440-44
ATCTCGT1094350.08.94924440-44
ACACGTC1316400.08.51007210-14
CGTCTGA1319300.08.50237615-19
CGTCTTC1390000.08.16906250-54
Produced by FastQC (version 0.11.5)