FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445537281
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1298100.040.3870969
GAGCGTC1658100.031.792378
ATCGGAA1871600.028.751431
TCGGAAG2006800.026.7859252
AGAGCGT2051000.025.9704917
CGGAAGA2251250.023.9602993
AAGAGCG2513100.021.4228236
CGCCGTA785050.013.73123555-59
TCGCCGT913800.011.84255755-59
GCCGTAT1034150.010.68296255-59
GAAGAGC5368950.010.599675
GTCGCCG1046150.010.47869855-59
CCGTATC1158250.09.42699555-59
TGGTCGC1200450.09.31058350-54
GGTCGCC1214050.09.00814550-54
GCGTCGT1204900.08.94507710-14
CGTATCA1258600.08.83542160-64
CGTCGTG1260750.08.60283110-14
GTGGTCG1320700.08.51332450-54
TCGTGTA1538000.07.24608610-14
Produced by FastQC (version 0.11.5)