FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455099314
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG10955400.240725478219464TruSeq Adapter, Index 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2404150.074.094661
TCGGAAG2573350.069.322062
CGGAAGA2788600.063.9685363
AGAGCAC5386250.033.621967
AGCACAC5665800.031.9174189
GAGCACA5809150.031.2129678
GAAGAGC5894100.030.8290215
AAGAGCA8438750.021.8505526
TATGCCG1701550.021.64183645-49
CTCGTAT1773550.020.04697640-44
CGTATGC1830850.019.90198945-49
TCTCGTA1823250.019.55061340-44
CGATAGA1865950.019.49426735-39
AGCGATA1887050.019.49053830-34
GGAAGAG9542400.019.4031374
GCGATAG1871250.019.128130-34
ATGCCGT1994700.018.51431545-49
TCGTATG1933000.017.98902540-44
GCCGTCT2057450.017.78434850-54
TGCCGTC2027950.017.7310445-49
Produced by FastQC (version 0.11.5)