FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455099314
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC11278270.24781997364206088Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2828100.063.6916059
GAGCGTC3209950.056.12858
ATCGGAA3412500.053.4610331
AGAGCGT3536950.051.152347
TCGGAAG3618800.050.2575452
CGGAAGA3755500.048.3916473
AAGAGCG4000750.045.3993156
GAAGAGC6905950.026.805675
CGCCGTA1901850.019.22700955-59
GGAAGAG10576200.017.9013774
TCGCCGT2092600.017.57270455-59
GCCGTAT2137250.017.2177955-59
GTCGCCG2270500.016.23348855-59
CCGTATC2256600.015.894917555-59
CGTATCA2315100.015.82779660-64
TGGTCGC2388450.015.53094850-54
GGTCGCC2445150.014.806334550-54
GTGGTCG2541650.014.6518250-54
GCGTCGT2760500.013.34797910-14
CGTCGTG2821350.013.06831310-14
Produced by FastQC (version 0.11.5)