FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474877266
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG11170720.23523383408293125TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2469300.073.889621
TCGGAAG2628450.069.356992
CGGAAGA2832700.064.219463
AGAGCAC5528650.033.258817
AGCACAC5857650.031.3350059
GAGCACA5987700.030.7960768
GAAGAGC6063150.030.4141545
TATGCCG1795000.022.14116145-49
AAGAGCA8727100.021.3960426
CTCGTAT1868000.020.61485540-44
CGTATGC1934300.020.30901745-49
TCTCGTA1932500.019.93280640-44
GGAAGAG9819350.019.2022114
ATGCCGT2086800.019.14102445-49
CGCTCAT2067900.019.07181230-34
TATCTCG2000800.018.95811340-44
ACCGCTC2157650.018.29664230-34
GCCGTCT2148150.018.28520650-54
GTCACCG2225450.017.9121225-29
ATCTCGT2174400.017.76331940-44
Produced by FastQC (version 0.11.5)