FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360673288
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG17379930.48187461002102266TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA5173050.055.328551
TCGGAAG5203700.055.0250362
CGGAAGA5334250.053.6380423
AGAGCAC7096200.040.5358057
AGCACAC7213200.039.7823529
GAGCACA7363550.039.0429238
GAAGAGC7667550.037.6334425
AAGAGCA9755700.029.7825766
GGAAGAG10590050.027.5098954
TATGCCG2790050.020.88107345-49
CGTATGC2940000.019.63014645-49
ATGCCGT2974350.019.55894545-49
GCCGTCT2944650.019.50145150-54
CTCGTAT2806000.019.41449440-44
TGCCGTC2939300.019.34477645-49
TCTCGTA2856250.019.06125840-44
TCGTATC3133400.018.62699735-39
CGTCTTC3117850.018.47346750-54
TATCTCG2953650.018.32714840-44
CCGTCTT3175600.018.28636450-54
Produced by FastQC (version 0.11.5)