FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438746674
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC7788840.1775247645524032Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2156400.054.8742759
GAGCGTC2512950.047.2728738
AGAGCGT2864250.041.6427047
ATCGGAA2911700.041.4616171
TCGGAAG2995200.040.2472272
CGGAAGA3216700.037.4960483
AAGAGCG3271050.036.6322566
CGCCGTA1192650.020.2654855-59
GAAGAGC6181100.019.8841975
TCGCCGT1295250.018.700555-59
GTCGCCG1383200.017.39403255-59
GCCGTAT1437600.016.96074755-59
TGGTCGC1512850.016.19942950-54
GGTCGCC1548750.015.53182850-54
CCGTATC1560050.015.45753255-59
GTGGTCG1618700.015.20371650-54
CGTATCA1638500.014.86723260-64
GTACGTC1656200.014.52986330-34
TACGTCC1739050.014.0310430-34
GGAAGAG9615400.013.2745274
Produced by FastQC (version 0.11.5)